Subcellular Localization of Asparaginase and Asparagine Aminotransferase in Pisum sativum Leaves.

Plant physiology ·Vol. 72 ·No. 4 ·1983-08-00 ·Pages 1127-9

Ireland RJ, Joy KW

Abstract

Protoplasts isolated from young and mature pea leaves (Pisum sativum L.) were broken and their contents fractionated by differential centrifugation or on sucrose-density gradients. Asparaginase was found only in the cytosol of young leaves. Asparagine aminotransferase was found in both young and mature leaves and was localized exclusively in the peroxisome. This corroborates the observation that asparagine transamination is catalyzed by the serine:glyoxylate aminotransferase.

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Ireland R J

Biology Department, Carleton University, Ottawa, Ontario K1S 5B6 Canada.

Joy K W

References (11)

11 references, click to expand

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Article Info

Journal

Plant physiology

Abbr.

Plant Physiol

ISSN

0032-0889

DOI

10.1104/pp.72.4.1127

Published

1983-08-00

Pages

1127-9

Language

English

Region

United States

NLM ID

0401224

PMCID

PMC1066386

External Links

PubMed source DOI: 10.1104/pp.72.4.1127 PMC Full Text View journal details

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Subcellular Localization of Asparaginase and Asparagine Aminotransferase in Pisum sativum Leaves.

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