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PMID: 16662622 Published · ppublish English Journal Article

High rates of protein synthesis by isolated chloroplasts.

Plant physiology ·Vol. 70 ·No. 4 ·1982-10-00 ·Pages 1107-14

Fish LE, Jagendorf AT

Abstract

Improvements are described in the preparation and in vitro conditions of an intact pea (Pisum sativum Progress No. 9) chloroplast system which provides high efficiency for translation of endogenous messenger RNA, using light as an energy source. High rates result in the incorporation into protein of up to 100 nanomoles tritiated leucine per milligram chlorophyll. These rates suggest extensive reinitiation, and repeated utilization of the messenger RNA that code for thylakoid proteins. Up to 39 radioactive thylakoid peptide bands were detected by fluorography after labeling with tritiated leucine.

Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Fish L E
Plant Biology Section, Cornell University, Ithaca, New York 14853.
Jagendorf A T
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Article Info
Journal
Plant physiology
Abbr.
Plant Physiol
ISSN
0032-0889
DOI
10.1104/pp.70.4.1107
Published
1982-10-00
Pages
1107-14
Language
English
Region
United States
NLM ID
0401224
PMCID
PMC1065834
External Links
PubMed source DOI: 10.1104/pp.70.4.1107 PMC Full Text View journal details
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