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PMID: 16661708 Published · ppublish English Journal Article

Similarity of Ribulose-1,5-bisphosphate Carboxylases of Isogenic Diploid and Tetraploid Ryegrass (Lolium perenne L.) Cultivars.

Plant physiology ·Vol. 67 ·No. 3 ·1981-03-00 ·Pages 530-4

McNeil PH, Foyer CH, Walker DA

Abstract

Partially purified ribulose-1,5-bisphosphate carboxylase (EC 4.1.1.39) was isolated from diploid and tetraploid cultivars of ryegrass (Lolium perenne L.) using two separate methods. The apparent K(m) (CO(2)) values for the enzymes prepared by either method did not differ significantly between diploid and tetraploid when assayed by two separate techniques. The unpurified enzymes from freshly lysed (and fully functional) protoplasts of both diploid and tetraploid cultivars gave virtually identical apparent K(m) (CO(2)) values. There was no indication of large differences in affinity for CO(2) of illuminated intact protoplasts from the two cultivars.

Authors & Affiliations
3 authors, click to expand affiliations / ORCID
McNeil P H
Agricultural Research Council Research Group on Photosynthesis, Botany Department, University of Sheffield, S10 2TN, United Kingdom.
Foyer C H
Walker D A
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Article Info
Journal
Plant physiology
Abbr.
Plant Physiol
ISSN
0032-0889
DOI
10.1104/pp.67.3.530
Published
1981-03-00
Pages
530-4
Language
English
Region
United States
NLM ID
0401224
PMCID
PMC425719
External Links
PubMed source DOI: 10.1104/pp.67.3.530 PMC Full Text View journal details
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