Translocation: EFFLUX OF SUGARS ACROSS THE PLASMALEMMA OF MESOPHYLL PROTOPLASTS.

Plant physiology ·Vol. 65 ·No. 3 ·1980-03-00 ·Pages 560-2

Huber SC, Moreland DE

Abstract

During photosynthesis by mesophyll protoplasts of wheat and tobacco, a linear efflux of sucrose and hexoses to the medium was observed, with the size of the intraprotoplast sugar pools remaining constant. Efflux of metabolites labeled by (14)CO(2) fixation was initially low because of dilution by internal pools, but increased exponentially with time. The results have significance both in terms of the mechanism of translocation and the use of isolated protoplasts in photosynthetic studies.

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Huber S C

United States Department of Agriculture, Science and Education Administration, Agricultural Research, Department of Crop Science, North Carolina State University, Raleigh, North Carolina 27650.

Moreland D E

References (9)

9 references, click to expand

Biochemical aspects of phloem transport.
Symp Soc Exp Biol. 1974;(28):43-62 PMID: 4616400
Enzymic assay of 10 to 10 moles of sucrose in plant tissues.
Plant Physiol. 1977 Sep;60(3):379-83 PMID: 16660097
Purification of enzymatically isolated mesophyll protoplasts from c(3), c(4), and crassulacean Acid metabolism plants using an aqueous dextran-polyethylene glycol two-phase system.
Plant Physiol. 1973 Nov;52(5):484-90 PMID: 16658589
Phloem Loading of Sucrose: pH Dependence and Selectivity.
Plant Physiol. 1977 Apr;59(4):750-5 PMID: 16659931
Metabolism of Separated Leaf Cells: III. Effects of Calcium and Ammonium on Product Distribution During Photosynthesis with Cotton Cells.
Plant Physiol. 1973 Jul;52(1):17-22 PMID: 16658491
Photosynthesis by isolated protoplasts, protoplast extracts, and chloroplasts of wheat: influence of orthophosphate, pyrophosphate, and adenylates.
Plant Physiol. 1978 Aug;62(2):313-9 PMID: 16660508
Vein Loading: The Role of the Symplast in Intercellular Transport of Carbohydrate between the Mesophyll and Minor Veins of Tobacco Leaves.
Plant Physiol. 1974 Jun;53(6):912-7 PMID: 16658815
Role of free space in translocation in sugar beet.
Plant Physiol. 1974 Dec;54(6):892-8 PMID: 16658995
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Article Info

Journal

Plant physiology

Abbr.

Plant Physiol

ISSN

0032-0889

DOI

10.1104/pp.65.3.560

Published

1980-03-00

Pages

560-2

Language

English

Region

United States

NLM ID

0401224

PMCID

PMC440377

External Links

PubMed source DOI: 10.1104/pp.65.3.560 PMC Full Text View journal details

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