Essential Sulfhydryl Group in the Transport-catalyzing Protein of the Hexose-Proton Cotransport System of Chlorella.

Plant physiology ·Vol. 61 ·No. 5 ·1978-05-00 ·Pages 785-6

Komor E, Weber H, Tanner W

Abstract

The polyene antibiotic nystatin transforms the sugar-proton contransport system of Chlorella to a mere facilitated diffusion system. This experimental condition was used to test the sugar-translocating unit of the active uptake system for possible essential sulfhydryl groups. It could be shown that the catalyzed translocation of sugar is sensitive to the sulfhydryl-reactive compound N-ethylmaleimide. Sugar flow by passive leak as induced by the detergent Triton X-100 is not affected by sulfhydryl reagents. These results show that the sugar-translocating carrier protein possesses a sulfhydryl group, which is essential for its function.

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Komor E

Botanik I, Fachbereich Biologie und Vorklinische Medizin Universität Regensburg, West Germany.

Weber H

Tanner W

References (16)

16 references, click to expand

The extraction, purification and some chemical properties of actin.
Biochim Biophys Acta. 1953 May;11(1):102-13 PMID: 13066460
Energy expenditure is obligatory for the downhill transport of galactosides.
J Mol Biol. 1971 Aug 14;59(3):447-59 PMID: 4937057
Glucose transport in plasma membrane vesicles from rat adipose tissue.
J Biol Chem. 1972 May 10;247(9):2682-8 PMID: 5025097
Evidence for Phloem loading from the apoplast: chemical modification of membrane sulfhydryl groups.
Plant Physiol. 1976 Jun;57(6):872-5 PMID: 16659588
The determination of the membrane ptoential of Chlorella vulgaris. Evidence for electrogenic sugar transport.
Eur J Biochem. 1976 Nov 1;70(1):197-204 PMID: 12943
Unusual features of the active hexose uptake system of Chlorella vulgaris.
Biochim Biophys Acta. 1972 Jun 20;266(3):649-60 PMID: 5040249
Transformation of a strictly coupled active transport system into a facilitated diffusion system by nystatin.
J Membr Biol. 1974 Jul 12;17(3):231-8 PMID: 4847760
The hexose-proton symport system of Chlorella vulgaris. Specificity, stoichiometry and energetics of sugar-induced proton uptake.
Eur J Biochem. 1974 May 2;44(1):219-23 PMID: 4854863
Specific labeling and partial purification of the M protein, a component of the beta-galactoside transport system of Escherichia coli.
Proc Natl Acad Sci U S A. 1965 Sep;54(3):891-9 PMID: 5324399
Sugar transport at the basal and lateral aspect of the small intestinal cell.
Biochim Biophys Acta. 1973 Mar 16;298(2):429-36 PMID: 4719140
Characterization and partial purification of an inducible protein related to hexose proton cotransport of Chlorella vulgaris.
Eur J Biochem. 1977 Feb;72(3):509-14 PMID: 837927
Sugar transport in immature internodal tissue of sugarcane: I. Mechanism and kinetics of accumulation.
Plant Physiol. 1972 Jan;49(1):82-6 PMID: 16657901
Sugar transport in the red blood cell: structure-activity relationships in substrates and antagonists.
Pharmacol Rev. 1961 Mar;13:39-70 PMID: 13760340
The essentiality of sulfhydryl groups to transport in Neurospora crassa.
Arch Biochem Biophys. 1975 Jun;168(2):483-9 PMID: 124553
Inability of diffusion to account for placental glucose transfer in the sheep and consideration of the kinetics of a possible carrier transfer.
J Physiol. 1952 Sep;118(1):23-39 PMID: 13000688
Sugar transport in Mycoplasma gallisepticum.
J Bacteriol. 1969 Feb;97(2):787-92 PMID: 5773029

Article Info

Journal

Plant physiology

Abbr.

Plant Physiol

ISSN

0032-0889

DOI

10.1104/pp.61.5.785

Published

1978-05-00

Pages

785-6

Language

English

Region

United States

NLM ID

0401224

PMCID

PMC1091977

External Links

PubMed source DOI: 10.1104/pp.61.5.785 PMC Full Text View journal details

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Essential Sulfhydryl Group in the Transport-catalyzing Protein of the Hexose-Proton Cotransport System of Chlorella.

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