Citric Acid cycle activity in mitochondria isolated from mung bean hypocotyls.

Plant physiology ·Vol. 58 ·No. 3 ·1976-09-00 ·Pages 426-32

Bowman EJ, Ikuma H, Stein HJ

Abstract

Citric acid cycle activity in mitochondria from mung bean (Phaseolus aureus var. Jumbo) hypocotyls were examined by surveying (a) characteristics of oxidation of cycle intermediates; (b) activities of cycle enzymes in mitochondrial extracts; (c) contents of cycle intermediates and electron transport components in isolated mitochondria; and (d) time-course changes of products formed during oxidation of succinate, malate, and citrate. Isolated mitochondria are deficient in thiamine pyro-phosphate and somewhat so in adenylates, but apparently sufficient in CoA, NAD, and electron transport carriers. Cycle activity in the mitochondria is not directly correlated with the activities of the enzymes measured in extracts. These studies led to the conclusion that the region between malate and citrate is an important regulatory area in citric acid cycle functioning in isolated mung bean mitochondria.

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Bowman E J

Department of Cellular and Molecular Biology, Division of Biological Sciences, University of Michigan, Ann Arbor, Michigan 48109.

Ikuma H

Stein H J

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21 references, click to expand

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Article Info

Journal

Plant physiology

Abbr.

Plant Physiol

ISSN

0032-0889

DOI

10.1104/pp.58.3.426

Published

1976-09-00

Pages

426-32

Language

English

Region

United States

NLM ID

0401224

PMCID

PMC542260

External Links

PubMed source DOI: 10.1104/pp.58.3.426 PMC Full Text View journal details

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Citric Acid cycle activity in mitochondria isolated from mung bean hypocotyls.

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