Fat Metabolism in Higher Plants: LXII. Stearl-acyl Carrier Protein Desaturase from Spinach Chloroplasts.

Plant physiology ·Vol. 54 ·No. 4 ·1974-10-00 ·Pages 484-6

Jacobson BS, Jaworski JG, Stumpf PK

Abstract

Stearyl-acyl carrier protein desaturase (EC 1.14.99.6), present in the stroma fraction of spinach (Spinacia oleracea) chloroplasts, rapidly desaturated enzymatically prepared stearyl-acyl carrier protein to oleic acid. No other substrates were desaturated. In addition to stearyl-acyl carrier protein, reduced ferredoxin was an essential component of the system. The electron donor systems were either ascorbate, dichlorophenolindophenol, photosystem I and light, or NADPH and ferredoxin-NADP reductase. The desaturase was more active in extracts prepared from chloroplasts obtained from immature spinach leaves than from mature leaves. Stearyl-acyl carrier protein desaturase also occurs in soluble extracts of avocado (Persea americana Mill.) mesocarp and of developing safflower (Carthamus tinctorius) seeds.

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Jacobson B S

Department of Biochemistry and Biophysics, University of California, Davis, California 95616.

Jaworski J G

Stumpf P K

References (12)

12 references, click to expand

Fat metabolism in higher plants. Properties of a soluble stearyl-acyl carrier protein desaturase from maturing Carthamus tinctorius.
Arch Biochem Biophys. 1974 May;162(1):158-65 PMID: 4831331
Desaturation of palmitate and stearate by cell-free fractions from soybean cotyledons.
Lipids. 1969 Nov;4(6):539-43 PMID: 5367937
A requirement for three protein components in microsomal stearyl coenzyme A desaturation.
Biochemistry. 1971 Apr 27;10(9):1556-60 PMID: 4325603
Fat metabolism in higher plants. Enzymatic preparation of E. coli stearyl-acyl carrier protein.
Arch Biochem Biophys. 1974 May;162(1):166-73 PMID: 4598528
Protein measurement with the Folin phenol reagent.
J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
Enzymatic desaturation of stearyl acyl carrier protein.
J Biol Chem. 1968 Sep 10;243(17):4626-33 PMID: 4300868
Factors involved in fatty acyl CoA desaturation by fungal microsomes. The relative roles of acyl CoA and phospholipids as substrates.
Eur J Biochem. 1971 Mar 11;19(2):200-10 PMID: 4324068
Enzymatic desaturation of stearyl acyl carrier protein.
J Biol Chem. 1966 Apr 25;241(8):1925-7 PMID: 5945865
Mössbauer effect in Scenedesmus and spinach ferredoxins. The mechanism of electron transfer in plant-type iron-sulphur proteins.
Biochem J. 1971 Apr;122(3):257-65 PMID: 4330196
Fat metabolism in higher plants. 48. Properties of oleyl coenzyme A desaturase of Carthamus tinctorius.
J Biol Chem. 1972 Jan 25;247(2):360-6 PMID: 4333259
Fat Metabolism in Higher Plants: LVII. A Comparison of Fatty Acid-Synthesizing Enzymes in Chloroplasts Isolated from Mature and Immature Leaves of Spinach.
Plant Physiol. 1973 Aug;52(2):156-61 PMID: 16658518
[A new pathway for oleic acid biosynthesis in plants].
Biochimie. 1973;55(11):1481-9 PMID: 4790853

Article Info

Journal

Plant physiology

Abbr.

Plant Physiol

ISSN

0032-0889

DOI

10.1104/pp.54.4.484

Published

1974-10-00

Pages

484-6

Language

English

Region

United States

NLM ID

0401224

PMCID

PMC367438

External Links

PubMed source DOI: 10.1104/pp.54.4.484 PMC Full Text View journal details

Analysis Services

Fat Metabolism in Higher Plants: LXII. Stearl-acyl Carrier Protein Desaturase from Spinach Chloroplasts.

Contact

WeChat Official Account