Carbon dioxide fixation in the light and in the dark by isolated spinach chloroplasts.

Plant physiology ·Vol. 53 ·No. 2 ·1974-02-00 ·Pages 140-3

Avron M, Gibbs M

Abstract

Factors affecting CO(2) fixation in the spinach (Spinacia oleracea) chloroplast were investigated. Free magnesium ions are shown to be highly inhibitory for photosynthetic CO(2) fixation in isolated intact spinach chloroplasts. The pH optimum for CO(2) fixation is about 8.5 but is dependent upon the reaction medium. Conditions are defined under which chloroplasts illuminated in the absence of CO(2) accumulate ribulose 1,5-diphosphate, and fix CO(2) in a subsequent dark period when high magnesium ion concentrations are provided. The regulation of photosynthetic CO(2) assimilation by these factors is discussed.

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Avron M

Department of Biology, Brandeis University, Waltham, Massachusetts 02154.

Gibbs M

References (11)

11 references, click to expand

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Article Info

Journal

Plant physiology

Abbr.

Plant Physiol

ISSN

0032-0889

DOI

10.1104/pp.53.2.140

Published

1974-02-00

Pages

140-3

Language

English

Region

United States

NLM ID

0401224

PMCID

PMC541352

External Links

PubMed source DOI: 10.1104/pp.53.2.140 PMC Full Text View journal details

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Carbon dioxide fixation in the light and in the dark by isolated spinach chloroplasts.

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