Mechanism of naphthaleneacetic Acid conjugation: no effect of ethylene.

Formation of naphthaleneacetic acid-glucose (NAGLu) in detached leaves, floating on alpha-naphthaleneacetic acid-1-(14)C (NAA, 0.05 microcurie per milliliter, 3.1 mum)-buffer solution (phosphate-citrate, pH 4.2) began immediately while there was a 2- to 4-hour lag before NAA-asparatate (NAAsp) could be detected. Subsequent increase in the NAAsp conjugate reflected a decrease in free NAA to 1 to 2% of the total radioactivity taken up. Pretreatment with 31 mum(12)C-NAA for 18 hours doubled NAAsp formation after transfer for 4 hours to (14)C-NAA. Pretreatment with ethylene, as ethephon (up to 400 milligrams per liter) or ethylene gas (10 microliters per liter), did not induce NAAsp formation. In the presence of NAA, ethylene had no effect on NAA conjugation. Similarly, CO(2) (5%) did not modify the formation of the conjugates. Rhizobitoxine (1.87 mum) inhibited NAA-induced ethylene production but did not prevent NAA-induced formation of NAAsp. We concluded that the conjugation of NAA with aspartic acid is not mediated by ethylene.