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PMID: 16657599 Published · ppublish English Journal Article

Isolation of Intact Chloroplasts from Euglena gracilis by Zonal Centrifugation.

Plant physiology ·Vol. 47 ·No. 2 ·1971-02-00 ·Pages 217-21

Vasconcelos A, Pollack M, Mendiola LR, Hoffmann HP, Brown DH, Price CA

Abstract

Chloroplasts were separated from Euglena gracilis by zonal centrifugation at low speed in density gradients of Ficoll or dextran. The chloroplasts were intact by the criteria of ultrastructure and their content of ribulose diphosphate carboxylase and soluble protein. The chloroplasts also contained ribosomes and ribosomal RNA uncontaminated by the corresponding cytoplasmic particles.

Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Vasconcelos A
Department of Biochemistry and Microbiology (CAES), Rutgers University, New Brunswick, New Jersey 08903.
Pollack M
Mendiola L R
Hoffmann H P
Brown D H
Price C A
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16 references, click to expand
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Article Info
Journal
Plant physiology
Abbr.
Plant Physiol
ISSN
0032-0889
DOI
10.1104/pp.47.2.217
Published
1971-02-00
Pages
217-21
Language
English
Region
United States
NLM ID
0401224
PMCID
PMC365845
External Links
PubMed source DOI: 10.1104/pp.47.2.217 PMC Full Text View journal details
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