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PMID: 16656943 Published · ppublish English Journal Article

Oxygen evolution and the permeability of the outer envelope of isolated whole chloroplasts.

Plant physiology ·Vol. 43 ·No. 10 ·1968-10-00 ·Pages 1597-604

Robinson JM, Stocking CR

Abstract

A rapid oxygraph method of studying the permeability of the envelope of isolated chloroplasts was used. The outer envelope of aqueously isolated whole spinach (Spinacia oleracea L.) chloroplasts in buffer is readily permeable to 3-phosphoglyceric acid, which induces an immediate light dependent oxygen evolution. This light dependent oxygen evolution was completely eliminated by swelling these plastids in an osmotically dilute solution. Exogenous adenosine diphosphate, but not inorganic phosphate, strongly stimulated this oxygen evolution. This indicated that the chloroplast envelope is relatively permeable to adenosine diphosphate.Oxygen evolution and swelling studies indicated that the chloroplast envelope is relatively impermeable to NADP and to ferredoxin.A method is described whereby the percent of whole chloroplasts present in a chloroplast preparation may be rapidly estimated.

Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Robinson J M
Department of Botany, University of California, Davis, California 95616.
Stocking C R
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20 references, click to expand
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Article Info
Journal
Plant physiology
Abbr.
Plant Physiol
ISSN
0032-0889
DOI
10.1104/pp.43.10.1597
Published
1968-10-00
Pages
1597-604
Language
English
Region
United States
NLM ID
0401224
PMCID
PMC1087047
External Links
PubMed source DOI: 10.1104/pp.43.10.1597 PMC Full Text View journal details
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