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PMID: 16644267 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Review

Phosphatidylinositol 3-phosphate recognition and membrane docking by the FYVE domain.

Biochimica et biophysica acta ·Vol. 1761 ·No. 8 ·2006-08-00 ·Pages 868-77

Kutateladze TG

Abstract

The FYVE domain is a small zinc binding module that recognizes phosphatidylinositol 3-phosphate [PtdIns(3)P], a phospholipid enriched in membranes of early endosomes and other endocytic vesicles. It is usually present as a single module or rarely as a tandem repeat in eukaryotic proteins involved in a variety of biological processes including endo- and exocytosis, membrane trafficking and phosphoinositide metabolism. A number of FYVE domain-containing proteins are recruited to endocytic membranes through the specific interaction of their FYVE domains with PtdIns(3)P. Structures and PtdIns(3)P binding modes of several FYVE domains have recently been characterized, shedding light on the molecular basis underlying multiple cellular functions of these proteins. Here, structural and functional aspects and the current mechanism of the multivalent membrane anchoring by monomeric or dimeric FYVE domain are reviewed. This mechanism involves stereospecific recognition of PtdIns(3)P that is facilitated by non-specific electrostatic contacts and modulated by the histidine switch, and is accompanied by hydrophobic insertion. Contributions of each component to the FYVE domain specificity and affinity for PtdIns(3)P-containing membranes are discussed.

MeSH Terms
Amino Acid Sequence Cell Membrane/chemistry,metabolism Dimerization Histidine/chemistry Humans Membrane Lipids/chemistry,metabolism Models, Biological Models, Molecular Molecular Sequence Data Phosphatidylinositol Phosphates/chemistry,metabolism Protein Binding Protein Structure, Tertiary Sensitivity and Specificity Sequence Homology, Amino Acid Static Electricity
Chemicals
Membrane Lipids Phosphatidylinositol Phosphates phosphatidylinositol 3-phosphate Histidine
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Kutateladze Tatiana G
Department of Pharmacology, University of Colorado Health Sciences Center, 12801 East 17th Avenue, Aurora, CO 80045, USA. tatiana.kutateladze@uchsc.edu
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Article Info
Journal
Biochimica et biophysica acta
Abbr.
Biochim Biophys Acta
ISSN
0006-3002
DOI
10.1016/j.bbalip.2006.03.011
Email
tatiana.kutateladze@uchsc.edu
Published
2006-08-00
Epub
2006-00-07
Pages
868-77
Language
English
Region
Netherlands
NLM ID
0217513
PMCID
PMC2740714
Subset
IM
Grants
NCI NIH HHS · R01 CA113472 · United States
NCI NIH HHS · R01 CA113472-03 · United States
NIGMS NIH HHS · R01 GM071424 · United States
NIGMS NIH HHS · R01 GM071424-03 · United States
External Links
PubMed source DOI: 10.1016/j.bbalip.2006.03.011 PMC Full Text
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