The Epstein-Barr virus BMRF1 gene is essential for lytic virus replication.

Journal of virology ·Vol. 80 ·No. 10 ·2006-05-00 ·Pages 5078-81

Neuhierl B, Delecluse HJ

Abstract

The Epstein-Barr virus (EBV) BMRF1 protein is a DNA polymerase processivity factor. We have deleted the BMRF1 open reading frame from the EBV genome and assessed the DeltaBMRF1 EBV phenotype. DeltaBMRF1 viruses were replication deficient, but the wild-type phenotype could be restored by BMRF1 trans-complementation. The replication-deficient phenotype included impaired lytic DNA replication and late protein expression. DeltaBMRF1 and wild-type viruses were undistinguishable in terms of their ability to transform primary B cells. Our results provide genetic evidence that BMRF1 is essential for lytic replication of the EBV genome.

MeSH Terms

Antigens, Viral/genetics,physiology B-Lymphocytes/metabolism,pathology Cell Transformation, Neoplastic/genetics,pathology Cell Transformation, Viral/genetics DNA Replication/genetics Gene Deletion Genome, Viral Herpesvirus 4, Human/genetics Humans Virus Replication/genetics

Chemicals

Antigens, Viral Epstein-Barr virus early antigen diffuse component

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Neuhierl Bernhard

German Cancer Research Center, Department of Virus Associated Tumours, Im Neuenheimer Feld 242, 69120 Heidelberg, Germany.

Delecluse Henri-Jacques

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Article Info

Journal

Journal of virology

Abbr.

J Virol

ISSN

0022-538X

DOI

10.1128/JVI.80.10.5078-5081.2006

Published

2006-05-00

Pages

5078-81

Language

English

Region

United States

NLM ID

0113724

PMCID

PMC1472063

Subset

External Links

PubMed source DOI: 10.1128/JVI.80.10.5078-5081.2006 PMC Full Text View journal details

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The Epstein-Barr virus BMRF1 gene is essential for lytic virus replication.

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