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PMID: 16637074 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Protocadherin-PC promotes androgen-independent prostate cancer cell growth.

The Prostate ·Vol. 66 ·No. 10 ·2006-07-01 ·Pages 1100-13

Terry S, Queires L, Gil-Diez-de-Medina S, Chen MW, de la Taille A, Allory Y, Tran PL, Abbou CC, Buttyan R, Vacherot F

Abstract

Protocadherin-PC (PCDH-PC) expression is upregulated in apoptosis-resistant sublines of the LNCaP human prostate cancer (CaP) cell line. Here, we assess the role of PCDH-PC in CaP cells and its mRNA expression in human prostate tissues. LNCaP cells transfected with PCDH-PC were tested for their ability to grow in vitro and in vivo in androgen-deprived conditions. PCDH-PC mRNA expression was evaluated by semi-quantitative RT-PCR and by in situ hybridization. PCDH-PC expression induced Wnt signaling in CaP cells and permitted androgen-independent growth of hormone-sensitive CaP cells. Expression of PCDH-PC-homologous transcripts was low and restricted to some epithelial cells in normal tissue and to CaP cells in tumors. However, hormone-resistant CaP cells expressed significantly higher levels of PCDH-PC-related mRNA. Our findings suggest a novel mechanism for the progression of CaP involving expression of PCDH-PC. This novel protocadherin induces Wnt signaling, promotes malignant behavior and hormone-resistance of CaP cells.

MeSH Terms
Androgens/physiology Animals Apoptosis/physiology Cadherins/analysis,genetics,physiology Cell Line, Tumor Cell Proliferation Gene Expression Regulation, Neoplastic Humans Immunohistochemistry In Situ Hybridization Male Mice Mice, Nude Neoplasms, Hormone-Dependent/chemistry,genetics,pathology,physiopathology Prostate/chemistry Prostatic Neoplasms/chemistry,genetics,pathology,physiopathology Protocadherins RNA, Messenger/analysis,genetics Reverse Transcriptase Polymerase Chain Reaction Signal Transduction/physiology TCF Transcription Factors/physiology Wnt Proteins/genetics,physiology Xenograft Model Antitumor Assays
Chemicals
Androgens Cadherins PCDH11Y protein, human Protocadherins RNA, Messenger TCF Transcription Factors Wnt Proteins
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Terry Stephane
Department of Urology and Pathology of CHU Henri Mondor, INSERM E 03-37, Université Paris XII, Centre de Recherches Chirurgicales, Assistance Publique des Hôpitaux de Paris, Créteil, France.
Queires Luis
Gil-Diez-de-Medina Sixtina
Chen Min-Wei
de la Taille Alexandre
Allory Yves
Tran Phuong-Lan
Abbou Claude C
Buttyan Ralph
Vacherot Francis
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Article Info
Journal
The Prostate
Abbr.
Prostate
ISSN
0270-4137
DOI
10.1002/pros.20446
Published
2006-07-01
Pages
1100-13
Language
English
Region
United States
NLM ID
8101368
PMCID
PMC2660890
Subset
IM
Grants
NCI NIH HHS · R01 CA111618 · United States
NCI NIH HHS · R01 CA111618-04 · United States
NCI NIH HHS · CA111618 · United States
External Links
PubMed source DOI: 10.1002/pros.20446 PMC Full Text View journal details
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