Home LiteratureArticle Details

PMID: 1663293 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

A second group of hepatitis C viruses.

Virus genes ·Vol. 5 ·No. 3 ·1991-07-00 ·Pages 243-54

Tsukiyama-Kohara K, Kohara M, Yamaguchi K, Maki N, Toyoshima A, Miki K, Tanaka S, Hattori N, Nomoto A

Abstract

cDNA clone 11-7 was isolated by immunoscreening a cDNA library that was prepared from a pooled plasma of non-A non-B hepatitis (NANBH) patients using expression vector lambda gt11. This cDNA corresponds to known nucleotide positions 3983-4745 of the genome of hepatitis C virus (HCV). This clone was used as a probe for screening the HCV-related cDNAs in a cDNA library similarly prepared by using lambda gt10. As a result, six more cDNA clones were isolated and analyzed for their nucleotide sequences. The results strongly suggested that there are at least two groups of HCV, group I and group II. According to our classification, the prototype HCV and clone 11-7 belong to group I HCV, and their nucleotide and deduced amino acid sequences were diverged from those of group II HCV. Genetic variation observed in the nucleotide and the amino acid sequences between the two groups resembles that in the NS3 region of the genome between Japanese encephalitis virus and West Nile fever virus. Polypeptides produced in Escherichia coli carrying a clone 11-7 or a group II cDNA clone E reacted with antibodies in the blood of 12 or 4 out of 14 individual chronic NANBH patients, respectively. Our data clearly indicate the existence of a second group of HCV.

MeSH Terms

Amino Acid Sequence Base Sequence DNA, Viral/genetics Gene Library Genes, Viral Hepacivirus/genetics Hepatitis C/genetics,immunology,microbiology Humans Molecular Sequence Data Mutation Polymerase Chain Reaction Sequence Homology, Nucleic Acid

Chemicals

DNA, Viral

Authors & Affiliations

9 authors, click to expand affiliations / ORCID

Tsukiyama-Kohara K

Fundamental Research Laboratory, Tonen Co., Saitama, Japan.

Kohara M

Yamaguchi K

Maki N

Toyoshima A

Miki K

Tanaka S

Hattori N

Nomoto A

References (22)

22 references, click to expand

Complete nucleotide sequences of all three poliovirus serotype genomes. Implication for genetic relationship, gene function and antigenic determinants.
J Mol Biol. 1984 Apr 25;174(4):561-85 PMID: 6202874
Hepatitis B virus, hepatitis non-A, non-B virus and hepatitis delta virus in lyophilized antihemophilic factor: relative sensitivity to heat.
Hepatology. 1985 Nov-Dec;5(6):1091-9 PMID: 3934060
Transmission of non-A, non-B hepatitis from man to chimpanzee.
Lancet. 1978 Mar 4;1(8062):463-6 PMID: 76018
Efficient isolation of genes by using antibody probes.
Proc Natl Acad Sci U S A. 1983 Mar;80(5):1194-8 PMID: 6219389
Hepatitis C virus shares amino acid sequence similarity with pestiviruses and flaviviruses as well as members of two plant virus supergroups.
Proc Natl Acad Sci U S A. 1990 Mar;87(6):2057-61 PMID: 2156259
Identification of mutations that occurred on the genome of Japanese encephalitis virus during the attenuation process.
Virus Genes. 1991 Apr;5(2):95-109 PMID: 1829286
Toga-like virus as a cause of fulminant hepatitis attributed to sporadic non-A, non-B.
J Med Virol. 1989 Jul;28(3):150-5 PMID: 2502604
Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.
Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350-4 PMID: 388439
An assay for circulating antibodies to a major etiologic virus of human non-A, non-B hepatitis.
Science. 1989 Apr 21;244(4902):362-4 PMID: 2496467
The 5'-terminal sequence of the hepatitis C virus genome.
Jpn J Exp Med. 1990 Jun;60(3):167-77 PMID: 2170712
Hepatitis C viral cDNA clones isolated from a healthy carrier donor implicated in post-transfusion non-A, non-B hepatitis.
Gene. 1990 Jul 16;91(2):287-91 PMID: 2170237
DNA sequencing with chain-terminating inhibitors.
Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
Isolation of a cDNA clone derived from a blood-borne non-A, non-B viral hepatitis genome.
Science. 1989 Apr 21;244(4902):359-62 PMID: 2523562
A cDNA fragment of hepatitis C virus isolated from an implicated donor of post-transfusion non-A, non-B hepatitis in Japan.
Nucleic Acids Res. 1989 Dec 25;17(24):10367-72 PMID: 2513560
Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase.
Science. 1988 Jan 29;239(4839):487-91 PMID: 2448875
Primary structure of the West Nile flavivirus genome region coding for all nonstructural proteins.
Virology. 1986 Feb;149(1):10-26 PMID: 3753811
Molecular cloning and complete nucleotide sequence of the genome of Japanese encephalitis virus Beijing-1 strain.
Virus Genes. 1988 Jun;1(3):305-17 PMID: 3245133
Complete nucleotide sequence of the Japanese encephalitis virus genome RNA.
Virology. 1987 Dec;161(2):497-510 PMID: 3686827
Non-A, non-B hepatitis: ultrastructural evidence for two agents in experimentally infected chimpanzees.
Science. 1979 Jul 13;205(4402):197-200 PMID: 451589
Persistent non-A, non-B hepatitis in experimentally infected chimpanzees.
J Infect Dis. 1981 Feb;143(2):210-8 PMID: 6783708
Posttransfusion non-A, non-B hepatitis in chimpanzees. Physicochemical evidence that the tubule-forming agent is a small, enveloped virus.
Gastroenterology. 1985 Mar;88(3):773-9 PMID: 2981754
Determining the size of non-A, non-B hepatitis virus by filtration.
J Infect Dis. 1987 Oct;156(4):636-40 PMID: 3114389

Article Info

Journal

Virus genes

Abbr.

Virus Genes

ISSN

0920-8569

DOI

10.1007/BF00568974

Published

1991-07-00

Pages

243-54

Language

English

Region

United States

NLM ID

8803967

Subset

External Links

PubMed source DOI: 10.1007/BF00568974 View journal details

Analysis Services

A second group of hepatitis C viruses.

Contact

WeChat Official Account