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PMID: 16627761 Published · ppublish English Journal Article Research Support, N.I.H., Intramural Research Support, Non-U.S. Gov't

Proprotein convertase furin is preferentially expressed in T helper 1 cells and regulates interferon gamma.

Blood ·Vol. 108 ·No. 3 ·2006-08-01 ·Pages 983-5

Pesu M, Muul L, Kanno Y, O'Shea JJ

Abstract

Interleukin 12 (IL-12) is a major inducer of interferon gamma (IFN-gamma) and the principal mediator of T helper 1 (Th1) differentiation. To identify IL-12-regulated genes, which might contribute to Th1 differentiation and IFNG regulation, we employed microarray analysis. Surprisingly, a ubiquitously expressed proprotein convertase (PC), furin, was one of the most consistently IL-12-induced genes in T cells, and among PCs was the only one regulated by this cytokine. Furin was preferentially expressed in differentiated Th1 cells in a Stat4-dependent manner. Expression of furin enhanced IFN-gamma secretion, whereas inhibition of furin interfered with IFN-gamma production. Thus, we conclude that IL-12 induction of furin might represent a new aspect of IFN-gamma regulation and control of Th1 differentiation.

MeSH Terms
Cell Differentiation/drug effects Furin/genetics,physiology Gene Expression Profiling Gene Expression Regulation/drug effects,immunology Humans Interferon-gamma/biosynthesis,metabolism Interleukin-12/pharmacology Oligonucleotide Array Sequence Analysis Proprotein Convertases/genetics,physiology STAT4 Transcription Factor/physiology Th1 Cells/metabolism
Chemicals
STAT4 Transcription Factor STAT4 protein, human Interleukin-12 Interferon-gamma Proprotein Convertases Furin
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Pesu Marko
MIIB-NIAMS-NIH, Bldg 10, Rm 9N262, 10 Center Dr, Bethesda, MD 20892, USA. pesum@mail.nih.gov
Muul Linda
Kanno Yuka
O'Shea John J
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Article Info
Journal
Blood
Abbr.
Blood
ISSN
0006-4971
DOI
10.1182/blood-2005-09-3824
Email
pesum@mail.nih.gov
Published
2006-08-01
Epub
2006-00-20
Pages
983-5
Language
English
Region
United States
NLM ID
7603509
PMCID
PMC1895858
Subset
IM
Grants
Intramural NIH HHS · United States
External Links
PubMed source DOI: 10.1182/blood-2005-09-3824 PMC Full Text View journal details
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