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PMID: 1662489 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Information from e.p.r. spectroscopy on the iron-sulphur centres of the iron-molybdenum protein (aldehyde oxidoreductase) of Desulfovibrio gigas.

The Biochemical journal ·Vol. 280 ( Pt 3) ·1991-12-15 ·Pages 817-20

Bray RC, Turner NA, Le Gall J, Barata BA, Moura JJ

Abstract

E.p.r. spectra of reduced iron-sulphur centres of the aldehyde oxidoreductase (iron-molybdenum protein) of Desulfovibrio gigas were recorded at X-band and Q-band frequencies and simulated. Results are consistent with the view that only two types of [2Fe-2S] clusters are present, as in eukaryotic molybdenum-containing hydroxylases. The data indicate the Fe/SI centre to be very similar, and the Fe/SII centre somewhat similar, to these centres in the eukaryotic enzymes.

MeSH Terms
Aldehyde Oxidoreductases/chemistry,isolation & purification Desulfovibrio/enzymology Electron Spin Resonance Spectroscopy Iron-Sulfur Proteins/chemistry,isolation & purification Molybdoferredoxin/chemistry
Chemicals
Iron-Sulfur Proteins Molybdoferredoxin Aldehyde Oxidoreductases
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Bray R C
School of Biological Sciences, University of Sussex, Brighton, U.K.
Turner N A
Le Gall J
Barata B A
Moura J J
References (27)
27 references, click to expand
  1. Identification of a molybdopterin-containing molybdenum cofactor in xanthine dehydrogenase from Pseudomonas aeruginosa.
    Biofactors. 1991 Jun;3(2):103-7 PMID: 1654922
  2. Studies by electron-paramagnetic-resonance spectroscopy on the mechanism of action of xanthine dehydrogenase from Veillonella alcalescens.
    Biochem J. 1976 Feb 1;153(2):287-95 PMID: 179532
  3. Novel aspects of the biochemistry of the molybdenum cofactor.
    Adv Enzymol Relat Areas Mol Biol. 1991;64:215-90 PMID: 2053467
  4. Enzymes depending on the pterin molybdenum cofactor: sequence families, spectroscopic properties of molybdenum and possible cofactor-binding domains.
    Biochim Biophys Acta. 1991 Mar 29;1057(2):157-85 PMID: 2015248
  5. Xanthine dehydrogenase and 2-furoyl-coenzyme A dehydrogenase from Pseudomonas putida Fu1: two molybdenum-containing dehydrogenases of novel structural composition.
    J Bacteriol. 1990 Oct;172(10):5999-6009 PMID: 2170335
  6. Isolation and characterization of a second molybdopterin dinucleotide: molybdopterin cytosine dinucleotide.
    Arch Biochem Biophys. 1990 Dec;283(2):542-5 PMID: 2275562
  7. Electron-paramagnetic-resonance studies using pre-steady-state kinetics and substitution with stable isotopes on the mechanism of action of molybdoenzymes.
    Biochem Soc Trans. 1985 Jun;13(3):560-7 PMID: 2993062
  8. The isolation of demolybdo xanthine oxidase from bovine milk.
    Biochem J. 1988 Nov 1;255(3):949-56 PMID: 2850803
  9. The molybdenum iron-sulphur protein from Desulfovibrio gigas as a form of aldehyde oxidase.
    Biochem J. 1987 May 1;243(3):755-61 PMID: 2821990
  10. Nucleotide sequence encoding the flavoprotein and iron-sulfur protein subunits of the Bacillus subtilis PY79 succinate dehydrogenase complex.
    J Bacteriol. 1987 Feb;169(2):864-73 PMID: 3027051
  11. Sequence of the structural gene for xanthine dehydrogenase (rosy locus) in Drosophila melanogaster.
    Genetics. 1987 May;116(1):67-73 PMID: 3036646
  12. Mutations affecting expression of the rosy locus in Drosophila melanogaster.
    Genetics. 1987 May;116(1):55-66 PMID: 3036645
  13. Spectroscopic studies on the iron-sulfur centers of milk xanthine oxidase.
    J Biol Chem. 1985 Sep 5;260(19):10569-75 PMID: 2993281
  14. The inorganic biochemistry of molybdoenzymes.
    Q Rev Biophys. 1988 Aug;21(3):299-329 PMID: 3065813
  15. Proteolytic conversion of xanthine dehydrogenase from the NAD-dependent type to the O2-dependent type. Amino acid sequence of rat liver xanthine dehydrogenase and identification of the cleavage sites of the enzyme protein during irreversible conversion by trypsin.
    J Biol Chem. 1990 Aug 25;265(24):14170-5 PMID: 2387845
  16. Molybdopterin guanine dinucleotide: a modified form of molybdopterin identified in the molybdenum cofactor of dimethyl sulfoxide reductase from Rhodobacter sphaeroides forma specialis denitrificans.
    Proc Natl Acad Sci U S A. 1990 Apr;87(8):3190-4 PMID: 2326278
  17. Optical and electron paramagnetic resonance spectroscopic studies on purine hydroxylase II from Aspergillus nidulans.
    Arch Biochem Biophys. 1984 Mar;229(2):596-603 PMID: 6322698
  18. Properties of the prosthetic groups of rabbit liver aldehyde oxidase: a comparison of molybdenum hydroxylase enzymes.
    Biochemistry. 1982 Jul 20;21(15):3561-8 PMID: 6288079
  19. Electron paramagnetic resonance properties and oxidation-reduction potentials of the molybdenum, flavin, and iron-sulfur centers of chicken liver xanthine dehydrogenase.
    Arch Biochem Biophys. 1980 May;201(2):468-75 PMID: 6249208
  20. Magnetic coupling of the molybdenum and iron-sulphur centres in xanthine oxidase and xanthine dehydrogenases.
    Biochem J. 1978 Mar 1;169(3):471-9 PMID: 25647
  21. Oxidation-reduction studies of the Mo-(2Fe-2S) protein from Desulfovibrio gigas.
    Biochem J. 1978 Aug 1;173(2):419-25 PMID: 212012
  22. A molybdenum-containing iron-sulphur protein from Desulphovibrio gigas.
    Biochem Biophys Res Commun. 1976 Oct 4;72(3):782-9 PMID: 186061
  23. Purification and properties of xanthine dehydroganase from Micrococcus lactilyticus.
    J Biol Chem. 1967 Sep 25;242(18):4108-17 PMID: 6061702
  24. Spin-spin interaction between molybdenum and one of the iron-sulphur systems of xanthine oxidase and its relevance to the enzymic mechanism.
    Biochem J. 1972 Nov;130(1):239-49 PMID: 4347785
  25. The preparation and properties of deflavo xanthine oxidase.
    J Biol Chem. 1969 Apr 10;244(7):1692-700 PMID: 4305460
  26. Studies by electron-paramagnetic-resonance spectroscopy and stopped-flow spectrophotometry on the mechanism of action of turkey liver xanthine dehydrogenase.
    Biochem J. 1976 Feb 1;153(2):297-307 PMID: 179533
  27. Crystallization and structure determination to 2.5-A resolution of the oxidized [2Fe-2S] ferredoxin isolated from Anabaena 7120.
    Biochemistry. 1991 Apr 30;30(17):4126-31 PMID: 1902376
Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj2800817
Published
1991-12-15
Pages
817-20
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1130529
Subset
IM
External Links
PubMed source DOI: 10.1042/bj2800817 PMC Full Text View journal details
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