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PMID: 16621811 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Methanocaldococcus jannaschii uses a modified mevalonate pathway for biosynthesis of isopentenyl diphosphate.

Journal of bacteriology ·Vol. 188 ·No. 9 ·2006-05-00 ·Pages 3192-8

Grochowski LL, Xu H, White RH

Abstract

Archaea have been shown to produce isoprenoids from mevalonate; however, genome analysis has failed to identify several genes in the mevalonate pathway on the basis of sequence similarity. A predicted archaeal kinase, coded for by the MJ0044 gene, was associated with other mevalonate pathway genes in the archaea and was predicted to be the "missing" phosphomevalonate kinase. The MJ0044-derived protein was tested for phosphomevalonate kinase activity and was found not to catalyze this reaction. The MJ0044 gene product was found to phosphorylate isopentenyl phosphate, generating isopentenyl diphosphate. Unlike other known kinases associated with isoprene biosynthesis, Methanocaldococcus jannaschii isopentenyl phosphate kinase is predicted to be a member of the aspartokinase superfamily.

MeSH Terms
Archaeal Proteins/metabolism Hemiterpenes/metabolism Methanococcaceae/metabolism Mevalonic Acid/metabolism Organophosphorus Compounds/metabolism Phosphotransferases/metabolism
Chemicals
Archaeal Proteins Hemiterpenes Organophosphorus Compounds isopentenyl pyrophosphate Phosphotransferases Mevalonic Acid
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Grochowski Laura L
Department of Biochemistry (0308), Virginia Polytechnic Institute and State University, Blacksburg, Virginia 24061, USA.
Xu Huimin
White Robert H
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/JB.188.9.3192-3198.2006
Published
2006-05-00
Pages
3192-8
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC1447442
Subset
IM
External Links
PubMed source DOI: 10.1128/JB.188.9.3192-3198.2006 PMC Full Text View journal details
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