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PMID: 16610002 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Up-regulation of NAD(P)H quinone oxidoreductase 1 during human liver injury.

World journal of gastroenterology ·Vol. 12 ·No. 12 ·2006-03-28 ·Pages 1937-40

Aleksunes LM, Goedken M, Manautou JE

Abstract

To investigate the expression and activity of NAD(P)H quinone oxidoreductase 1 (NQO1) in human liver specimens obtained from patients with liver damage due to acetaminophen (APAP) overdose or primary biliary cirrhosis (PBC). NQO1 activity was determined in cytosol from normal, APAP and PBC liver specimens. Western blot and immunohistochemical staining were used to determine patterns of NQO1 expression using a specific antibody against NQO1. NQO1 protein was very low in normal human livers. In both APAP and PBC livers, there was strong induction of NQO1 protein levels on Western blot. Correspondingly, significant up-regulation of enzyme activity (16- and 22-fold, P< 0.05) was also observed in APAP and PBC livers, respectively. Immunohistochemical analysis highlighted injury-specific patterns of NQO1 staining in both APAP and PBC livers. These data demonstrate that NQO1 protein and activity are markedly induced in human livers during both APAP overdose and PBC. Up-regulation of this cytoprotective enzyme may represent an adaptive stress response to limit further disease progression by detoxifying reactive species.

MeSH Terms

Acetaminophen/adverse effects Adolescent Adult Analgesics, Non-Narcotic/adverse effects Female Gene Expression Regulation, Enzymologic Humans Immunohistochemistry Liver/enzymology,pathology Liver Cirrhosis, Biliary/complications Liver Diseases/enzymology,etiology Male Middle Aged NAD(P)H Dehydrogenase (Quinone)/genetics Up-Regulation

Chemicals

Analgesics, Non-Narcotic Acetaminophen NAD(P)H Dehydrogenase (Quinone) NQO1 protein, human

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Aleksunes Lauren M

Department of Pharmaceutical Sciences, School of Pharmacy, University of Connecticut, 69 North Eagleville Road, Unit 3092, Storrs, CT 06269-3092, USA.

Goedken Michael

Manautou José E

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Article Info

Journal

World journal of gastroenterology

Abbr.

World J Gastroenterol

ISSN

1007-9327

DOI

10.3748/wjg.v12.i12.1937

Published

2006-03-28

Pages

1937-40

Language

English

Region

United States

NLM ID

100883448

PMCID

PMC4087521

Subset

External Links

PubMed source DOI: 10.3748/wjg.v12.i12.1937 PMC Full Text View journal details

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Up-regulation of NAD(P)H quinone oxidoreductase 1 during human liver injury.

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