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PMID: 16594071 Published · ppublish English Journal Article

Wounding induces the first enzyme of the shikimate pathway in Solanaceae.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 86 ·No. 19 ·1989-10-00 ·Pages 7370-3

Dyer WE, Henstrand JM, Handa AK, Herrmann KM

Abstract

The first enzyme of the shikimate pathway, 3-deoxy-D-arabino-heptulosonate-7-phosphate synthase (EC 4.1.2.15), is induced by wounding potato or tomato tissue. The increase in enzyme activity is associated with elevated amounts of the enzyme as determined by immunoblots. The specific wound-induced protein synthesis is preceded by an increase in the mRNA encoding this enzyme. The induced mRNA of potato tuber, leaf, and stem tissue is translated into a precursor polypeptide that is recognized by antibodies raised against the mature enzyme from tuber plastids. Wounding also induces mRNA encoding phenylalanine ammonia-lyase (EC 4.3.1.5), a key enzyme of plant secondary metabolism. The time courses for the induction of the two enzymes are similar, suggesting coordinate regulation for the biosynthesis of primary and secondary aromatic compounds.

Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Dyer W E
Department of Horticulture, Purdue University, West Lafayette, IN 47907.
Henstrand J M
Handa A K
Herrmann K M
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.86.19.7370
Published
1989-10-00
Pages
7370-3
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC298063
External Links
PubMed source DOI: 10.1073/pnas.86.19.7370 PMC Full Text View journal details
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