Identification of a unique isoform of 1-aminocyclopropane-1-carboxylic acid synthase by monoclonal antibody.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 85 ·No. 23 ·1988-12-00 ·Pages 8810-4

Mehta AM, Jordan RL, Anderson JD, Mattoo AK

Abstract

1-Aminocyclopropane-1-carboxylic acid (ACC) synthase (EC 4.4.1.14) is a key enzyme regulating ethylene biosynthesis in higher plants. A monoclonal antibody (mAb T20C) that immunoprecipitates the ACC synthase activity from tomato pericarp tissue extracts revealed that mAb T20C immunodecorates an approximately 67-kDa polypeptide. On isoelectric focusing gels, ACC synthase activity in cell-free preparations was resolved into three distinct activity peaks with pI values 5.3, 7, and 9. mAb T20C specifically recognized the pI 7 form of the enzyme on electrophoretic transfer (Western) blots. When analyzed by sodium dodecyl sulfate gel electrophoresis under reducing conditions, the eluted pI 7 form was confirmed to migrate as a polypeptide of 67 kDa. The 67-kDa pI 7 isoform is a previously undescribed form of ACC synthase.

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Mehta A M

Beltsville Agricultural Research Center, U.S. Department of Agriculture, Agricultural Research Service, Beltsville, MD 20705.

Jordan R L

Anderson J D

Mattoo A K

References (12)

12 references, click to expand

Radiolabeling of a wound-inducible pyridoxal phosphate-utilizing enzyme: evidence for its identification as ACC synthase.
Arch Biochem Biophys. 1987 Mar;253(2):333-40 PMID: 3566279
Ultrasensitive stain for proteins in polyacrylamide gels shows regional variation in cerebrospinal fluid proteins.
Science. 1981 Mar 27;211(4489):1437-8 PMID: 6162199
A quantitative dot-immunobinding assay for proteins using nitrocellulose membrane filters.
Proc Natl Acad Sci U S A. 1984 Mar;81(6):1684-7 PMID: 6424121
Monoclonal antibodies against HLA products and their use in immunoaffinity purification.
Methods Enzymol. 1983;92:110-38 PMID: 6343761
Use of monoclonal antibodies in the purification and characterization of 1-aminocyclopropane-1-carboxylate synthase, an enzyme in ethylene biosynthesis.
Proc Natl Acad Sci U S A. 1986 Oct;83(20):7755-9 PMID: 16593770
Ethylene-regulated gene expression: molecular cloning of the genes encoding an endochitinase from Phaseolus vulgaris.
Proc Natl Acad Sci U S A. 1986 Sep;83(18):6820-4 PMID: 2428042
A simple and sensitive assay for 1-aminocyclopropane-1-carboxylic acid.
Anal Biochem. 1979 Nov 15;100(1):140-5 PMID: 543532
Electrophoretic analysis of the major polypeptides of the human erythrocyte membrane.
Biochemistry. 1971 Jun 22;10(13):2606-17 PMID: 4326772
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
Anal Biochem. 1976 May 7;72:248-54 PMID: 942051
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
The rapidly metabolized 32,000-dalton polypeptide of the chloroplast is the "proteinaceous shield" regulating photosystem II electron transport and mediating diuron herbicide sensitivity.
Proc Natl Acad Sci U S A. 1981 Mar;78(3):1572-6 PMID: 6940173
Isolation of pure IgG1, IgG2a and IgG2b immunoglobulins from mouse serum using protein A-sepharose.
Immunochemistry. 1978 Jul;15(7):429-36 PMID: 30693

Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.85.23.8810

Published

1988-12-00

Pages

8810-4

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC282596

External Links

PubMed source DOI: 10.1073/pnas.85.23.8810 PMC Full Text View journal details

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Identification of a unique isoform of 1-aminocyclopropane-1-carboxylic acid synthase by monoclonal antibody.

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