Light-harvesting chlorophyll a/b-protein: Three-dimensional structure of a reconstituted membrane lattice in negative stain.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 82 ·No. 2 ·1985-01-00 ·Pages 386-90

Li J

Abstract

The three-dimensional structure of a negatively stained hexagonal membrane lattice containing the light-harvesting chlorophyll a/b-protein complex and phospholipids has been determined to 30-A resolution by image reconstruction from electron micrographs. This lattice has p321 symmetry, a lattice constant of 125 A and a thickness of 75 A. The monomer is shown to be an elongated molecule about 65 A long in the dimension perpendicular to the plane of the membrane. It spans the hydrophobic domain of the membrane in an asymmetric fashion, projecting [unk]20 A from one surface and less from the other. On the basis of this image and available biochemical data, the structure of the complex in the native thylakoid membrane is proposed.

Authors & Affiliations

1 authors, click to expand affiliations / ORCID

Li J

Department of Biochemistry, Columbia University, New York, NY 10032.

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29 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.82.2.386

Published

1985-01-00

Pages

386-90

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC397043

External Links

PubMed source DOI: 10.1073/pnas.82.2.386 PMC Full Text View journal details

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Light-harvesting chlorophyll a/b-protein: Three-dimensional structure of a reconstituted membrane lattice in negative stain.

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