Properties of a cell-free system for synthesis of citrus exocortis viroid.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 79 ·No. 20 ·1982-10-00 ·Pages 6285-8

Flores R, Semancik JS

Abstract

Partially purified nuclei from citrus exocortis viroid (CEV)-infected Gynura aurantiaca are able to synthesize linear and circular viroid molecules. Pretreatment of the nuclei with actinomycin D or deoxyribonuclease did not affect viroid synthesis, whereas the synthesis of other cellular RNAs was severely reduced. These observations support the essential role of CEV complementary RNA sequences in viroid replication. However, when alpha-amanitin was included in this in vitro synthesis system, CEV replication was markedly reduced by concentrations of 10 nM or greater. Taken together, these data support the proposition that viroid synthesis is catalyzed by a DNA-dependent RNA polymerase acting on a RNA template.

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Flores R

Department of Plant Pathology and Cell Interaction Research Group, University of California, Riverside, California 92521.

Semancik J S

References (13)

13 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.79.20.6285

Published

1982-10-00

Pages

6285-8

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC347105

External Links

PubMed source DOI: 10.1073/pnas.79.20.6285 PMC Full Text View journal details

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Properties of a cell-free system for synthesis of citrus exocortis viroid.

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