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PMID: 16593190 Published · ppublish English Journal Article

Tumor induction by Agrobacterium rhizogenes involves the transfer of plasmid DNA to the plant genome.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 79 ·No. 10 ·1982-05-00 ·Pages 3193-7

White FF, Ghidossi G, Gordon MP, Nester EW

Abstract

The DNA from tumors of Nicotiana glauca initiated by strains of Agrobacterium rhizogenes was shown to contain sequences that are homologous to the root-inducing (Ri) plasmid of the bacterium. Two independently established tumor lines contained a similar portion of the Ri-plasmid. The Ri-plasmid also hybridized to DNA fragments from uninfected N. glauca. A cosmid clone of the Ri-plasmid encompassing the region containing the Ri-plasmid sequences that are stably transferred to the plant also hybridized to the Ri-plasmid-related fragments found in uninfected plants. Five of six tumor lines tested produced a tumor-specific compound that is similar to agropine.

Authors & Affiliations
4 authors, click to expand affiliations / ORCID
White F F
Department of Microbiology and Immunology, University of Washington, Seattle, Washington 98195.
Ghidossi G
Gordon M P
Nester E W
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.79.10.3193
Published
1982-05-00
Pages
3193-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC346381
External Links
PubMed source DOI: 10.1073/pnas.79.10.3193 PMC Full Text View journal details
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