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PMID: 16592679 Published · ppublish English Journal Article

Iron-dextran antibody conjugates: General method for simultaneous staining of two components in high-resolution immunoelectron microscopy.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 76 ·No. 7 ·1979-07-00 ·Pages 3392-6

Dutton AH, Tokuyasu KT, Singer SJ

Abstract

We report the preparation and properties of an electron-dense antibody conjugate, made by the covalent bonding of an iron-dextran (Imposil) particle to an antibody molecule. Transmission electron microscopic experiments with the Imposil-antibody conjugates demonstrate their suitability as specific immunostains at high resolution, particularly for simultaneous double staining experiments in conjunction with ferritin-antibody conjugates.

Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Dutton A H
Department of Biology, University of California at San Diego, La Jolla, California 92093.
Tokuyasu K T
Singer S J
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.76.7.3392
Published
1979-07-00
Pages
3392-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC383831
External Links
PubMed source DOI: 10.1073/pnas.76.7.3392 PMC Full Text View journal details
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