RNA-dependent RNA polymerase of tobacco plants.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 75 ·No. 5 ·1978-05-00 ·Pages 2122-4

Ikegami M, Fraenkel-Conrat H

Abstract

Several properties of RNA polymerase (nucleosidetriphosphate:RNA nucleotidyltransferase, EC 2.7.7.6) active fractions obtained from tobacco plants, be they uninfected, infected with tobacco mosaic virus, or infected with tobacco necrosis virus, were compared. By the seven criteria tested, the RNA-dependent RNA polymerase from these three sources behaved the same, although its activity is greatly but variably stimulated by the two virus infections. It thus appears probable that these two viruses do not code for this enzyme, but rely for their replication on their ability to stimulate production of a host enzyme. The conclusion that cells contain RNA replicating capability represents a modification of the central dogma of molecular biology.

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Ikegami M

Department of Molecular Biology, University of California, Berkeley, California 94720.

Fraenkel-Conrat H

References (12)

12 references, click to expand

RNA polymerase from tobacco necrosis virus-infected and uninfected tobacco. II. Properties of the bound and soluble polymerases and the nature of their products.
Virology. 1977 Sep;81(2):224-36 PMID: 898658
RNA-dependent RNA polymerases in uninfected and in alfalfa mosaic virus-infected tobacco plants.
Virology. 1977 Oct 1;82(1):48-62 PMID: 898679
Cucumber mosaic virus-induced RNA polymerase: partial purification and properties of the template-free enzyme.
Virology. 1974 Dec;62(2):434-43 PMID: 4372786
Reticulocyte RNA-dependent RNA polymerase.
Proc Natl Acad Sci U S A. 1973 Dec;70(12):3400-4 PMID: 4519633
RNA-dependent RNA synthesis in rat brain.
Nature. 1974 May 31;249(456):445-8 PMID: 4599598
Characterization and specificity of soluble RNA polymerase of brome mosaic virus.
Virology. 1973 Apr;52(2):363-72 PMID: 4705381
In vitro synthesis of double-stranded RNA by an enzyme system isolated from tobacco leaves.
Biochim Biophys Acta. 1973 Aug 10;319(1):62-71 PMID: 4733693
Possible identification of a protein in brome mosaic virus infected barley as a component of viral RNA polymerase.
Virology. 1973 Jul;54(1):190-8 PMID: 4713912
Host and virus specific RNA polymerases in alfalfa mosaic virus infected tobacco.
Ann Microbiol (Paris). 1976 Jan;127A(1):183-92 PMID: 1275394
Rapid, multisample isoelectric focusing in sucrose density gradients using conventional polyacrylamide electrophoresis equpiment: a two-peak transient in the approach-to-equilibrium.
Anal Biochem. 1975 Nov;69(1):1-9 PMID: 2028
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
Anal Biochem. 1976 May 7;72:248-54 PMID: 942051
RNA polymerase from tobacco necrosis virus infected and uninfected tobacco. Purification of the membrane-associated enzyme.
Virology. 1976 Jul 1;72(1):23-32 PMID: 936480

Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.75.5.2122

Published

1978-05-00

Pages

2122-4

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC392503

External Links

PubMed source DOI: 10.1073/pnas.75.5.2122 PMC Full Text View journal details

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RNA-dependent RNA polymerase of tobacco plants.

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