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PMID: 1658800 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Genomic structure of the human prototype strain H of hepatitis C virus: comparison with American and Japanese isolates.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 88 ·No. 22 ·1991-11-15 ·Pages 10292-6

Inchauspe G, Zebedee S, Lee DH, Sugitani M, Nasoff M, Prince AM

Abstract

Genomic RNA from the human prototype strain H of the hepatitis C virus (HCV-H) has been molecularly cloned and sequenced. The HCV-H sequence reported consists of 9416 nucleotides including the 5' and 3' untranslated regions. HCV-H shows 96% amino acid identity with the American isolate HCV-1 but only 84.9% with the Japanese isolates HCV-J and HCV-BK. In addition to the hypervariable region (region V) previously identified in the putative E2 domain, three other variable domains were identified: region V1 (putative E1), region V2 (putative E2), and region V3 (putative NS5). These regions appear rather conserved (86-100%) among the American isolates (HCV-1 and HC-J1) or among various Japanese isolates (HCV-J, HCV-BK, HCV-JH, and HC-J4) but show striking heterogeneity when the two subgroups are compared (42-87.5% amino acid difference). A structural similarity between the 5'-terminal hairpin structure of HCV and of poliovirus was observed. This study further suggests the existence of at least two genomic subtypes of HCV and confirms a distant relationship between HCV and pestiviruses.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Cloning, Molecular DNA, Viral/genetics,isolation & purification Genetic Variation Genome, Viral Hepacivirus/genetics,isolation & purification Hepatitis C/microbiology Humans Japan Liver/microbiology Molecular Sequence Data Nucleic Acid Conformation Pan troglodytes Poliovirus/genetics Polymerase Chain Reaction RNA, Viral/genetics,isolation & purification Sequence Homology, Nucleic Acid United States
Chemicals
DNA, Viral RNA, Viral
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Inchauspe G
Lindsley F. Kimball Research Institute, New York Blood Center, Laboratory of Virology and Parasitology, NY 10021.
Zebedee S
Lee D H
Sugitani M
Nasoff M
Prince A M
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.88.22.10292
Published
1991-11-15
Pages
10292-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC52914
Subset
IM
Databases
GENBANK
M67463, M80719, M80720, M80721, M80722, M80723, M80724, S66591, S66593, S66727
External Links
PubMed source DOI: 10.1073/pnas.88.22.10292 PMC Full Text View journal details
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