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PMID: 16585651 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Prevalence of Bacteroides and Prevotella spp. in ulcerative colitis.

Journal of medical microbiology ·Vol. 55 ·No. Pt 5 ·2006-05-00 ·Pages 617-624

Lucke K, Miehlke S, Jacobs E, Schuppler M

Abstract

The resident bacterial flora of the large intestine has become increasingly recognized as an essential component in the pathogenesis of ulcerative colitis (UC). However, it is still not known whether the bacterial flora in general or certain bacterial species of the intestinal microbial flora contribute to the pathogenesis of the disease. In order to investigate the composition of the mucosa-associated microbial flora in UC, mucosal tissue samples from patients with active UC and from control subjects with non-inflammatory conditions were analysed and compared. To cover the whole spectrum of intestinal bacteria and to circumvent the known bias introduced by culture-based techniques, comparative 16S rRNA gene sequence analysis was used to determine the bacterial composition in the mucosal tissue samples. The investigation revealed an abundance of sequences from Bacteroides spp. and Prevotella spp. in the mucosal tissue of patients with UC compared with individuals showing no signs of disease. The higher incidence of populations of members of the Bacteroidetes in UC suggests that these may have an influence on the pathogenesis of the disease.

MeSH Terms

Bacteroides/classification,isolation & purification Biopsy Colitis, Ulcerative/etiology,microbiology Colonoscopy DNA, Bacterial/chemistry,genetics DNA, Ribosomal/chemistry,genetics Humans Intestinal Mucosa/microbiology Molecular Sequence Data Prevotella/classification,isolation & purification RNA, Ribosomal, 16S/genetics Sequence Analysis, DNA

Chemicals

DNA, Bacterial DNA, Ribosomal RNA, Ribosomal, 16S

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Lucke Katja

Institute of Medical Microbiology, Canton Hospital Luzern, Switzerland.

Miehlke Stephan

Medical Department I, University Hospital Dresden, Germany.

Jacobs Enno

Institute of Medical Microbiology and Hygiene, Technical University Dresden, Germany.

Schuppler Markus

Laboratory of Food Microbiology, Institute of Food Science and Nutrition, Swiss Federal Institute of Technology Zurich, ETH Center LFV B21, Schmelzbergstrasse 7, CH-8092 Zurich, Switzerland.

Article Info

Journal

Journal of medical microbiology

Abbr.

J Med Microbiol

ISSN

0022-2615

DOI

10.1099/jmm.0.46198-0

Published

2006-05-00

Pages

617-624

Language

English

Region

England

NLM ID

0224131

Subset

Databases

GENBANK

AJ812137, AJ812138, AJ812139, AJ812140, AJ812141, AJ812142, AJ812143, AJ812144, AJ812145, AJ812146, AJ812147, AJ812148, AJ812149, AJ812150, AJ812151, AJ812152, AJ812153, AJ812154, AJ812155, AJ812156, AJ812157, AJ812158, AJ812159, AJ812160, AJ812161, AJ812162, AJ812163, AJ812164, AJ812165, AJ812166, AJ812167, AJ812168, AJ812169, AJ812170, AJ812171, AJ812172, AJ812173, AJ812174, AJ812175, AJ812176, AJ812177, AJ812178, AJ812179, AJ812180, AJ812181, AJ812182, AJ812183, AJ812184, AJ812185, AJ812186, AJ812187, AJ812188, AJ812189, AJ812190, AJ812191, AJ812192, AJ812193, AJ812194, AJ812195, AJ812196, AJ812197, AJ812198, AJ812199, AJ812200, AJ812201, AJ812202, AJ812203, AJ812204, AJ812205, AJ812206, AJ812207, AJ812208, AJ812209, AJ812210, AJ812211, AJ812212

External Links

PubMed source DOI: 10.1099/jmm.0.46198-0 View journal details

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Prevalence of Bacteroides and Prevotella spp. in ulcerative colitis.

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