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PMID: 1657244 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Comparison of the primary sequences of two potato tuber ADP-glucose pyrophosphorylase subunits.

Plant molecular biology ·Vol. 17 ·No. 5 ·1991-11-00 ·Pages 1089-93

Nakata PA, Greene TW, Anderson JM, Smith-White BJ, Okita TW, Preiss J

Abstract

Near-full-length cDNA clones to the small and large subunit of the heterotetrameric potato tuber ADP-glucose pyrophosphorylase have been isolated and characterized. The missing amino terminal sequence of the small subunit has also been elucidated from its corresponding genomic clone. Primary sequence comparisons revealed that each potato subunit had less identity to each other than to their homologous subunit from other plants. It also appeared that the smaller subunit is more conserved among the different plants and the larger subunit more divergent. Amino acid comparisons of both potato tuber sequences to the Escherichia coli ADP-glucose pyrophosphorylase sequence revealed conserved regions important for both catalytic and allosteric function of the bacterial enzyme.

MeSH Terms
Amino Acid Sequence Cloning, Molecular DNA/genetics,isolation & purification Escherichia coli/enzymology,genetics Glucose-1-Phosphate Adenylyltransferase Macromolecular Substances Molecular Sequence Data Nucleotidyltransferases/genetics Plants/enzymology,genetics Sequence Homology, Nucleic Acid Solanum tuberosum/enzymology,genetics
Chemicals
Macromolecular Substances DNA Nucleotidyltransferases Glucose-1-Phosphate Adenylyltransferase
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Nakata P A
Program in Biochemistry and Biophysics, Washington State University, Pullman 99164-6340.
Greene T W
Anderson J M
Smith-White B J
Okita T W
Preiss J
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Article Info
Journal
Plant molecular biology
Abbr.
Plant Mol Biol
ISSN
0167-4412
DOI
10.1007/BF00037149
Published
1991-11-00
Pages
1089-93
Language
English
Region
Netherlands
NLM ID
9106343
Subset
IM
Databases
GENBANK
UNKNOWN
External Links
PubMed source DOI: 10.1007/BF00037149 View journal details
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