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PMID: 1656869 Published · ppublish English Comparative Study Journal Article

Quinolone resistance-determining region in the DNA gyrase gyrB gene of Escherichia coli.

Antimicrobial agents and chemotherapy ·Vol. 35 ·No. 8 ·1991-08-00 ·Pages 1647-50

Yoshida H, Bogaki M, Nakamura M, Yamanaka LM, Nakamura S

Abstract

Thirteen spontaneous quinolone-resistant gyrB mutants of Escherichia coli KL16, including two that were examined previously, were divided into two types according to their quinolone resistance patterns. Type 1 mutants were resistant to all the quinolones tested, while type 2 mutants were resistant to acidic quinolones and were hypersusceptible to amphoteric quinolones. Nucleotide sequence analysis disclosed that all nine type 1 mutants had a point mutation from aspartic acid to asparagine at amino acid 426 and that all four type 2 mutants had a point mutation from lysine to glutamic acid at amino acid 447.

Related Genes
gyrB
MeSH Terms
Amino Acid Sequence Binding Sites Chemical Phenomena Chemistry, Physical DNA Topoisomerases, Type II/chemistry,genetics,metabolism DNA, Bacterial/metabolism Drug Resistance, Microbial/genetics Escherichia coli/enzymology,genetics Molecular Sequence Data Mutation/genetics Quinolones/pharmacology
Chemicals
DNA, Bacterial Quinolones DNA Topoisomerases, Type II
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Yoshida H
Research Laboratories, Dainippon Pharmaceutical Co., Ltd., Osaka, Japan.
Bogaki M
Nakamura M
Yamanaka L M
Nakamura S
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Article Info
Journal
Antimicrobial agents and chemotherapy
Abbr.
Antimicrob Agents Chemother
ISSN
0066-4804
DOI
10.1128/AAC.35.8.1647
Published
1991-08-00
Pages
1647-50
Language
English
Region
United States
NLM ID
0315061
PMCID
PMC245234
Subset
IM
External Links
PubMed source DOI: 10.1128/AAC.35.8.1647 PMC Full Text View journal details
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