Assay for the Killing Properties of T2 Bacteriophage and Their "Ghosts".

Journal of bacteriology ·Vol. 90 ·No. 3 ·1965-09-00 ·Pages 724-8

Duckworth DH, Bessman MJ

Abstract

Duckworth, Donna H. (Johns Hopkins University, Baltimore, Md.), and Maurice J. Bessman. Assay for the killing properties of T2 bacteriophage and their "ghosts." J. Bacteriol. 90:724-728. 1965.-A procedure for the assay of bacteriophage and their "ghosts" which is based on their ability to kill cells is described. The method is derived from the well-known ability of phage and ghosts to prevent the induction of beta-galactosidase. Conditions are described whereby a direct relationship is found between the decrease in beta-galactosidase and the number of phage or ghosts present during the induction period. The number of phage measured by this method was found to be identical with the number of plaque-forming units found in a fresh lysate. The method has been used to follow the fate of ghosts under several conditions and to measure killer (but nonviable) particles in various preparations of phage.

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Duckworth D H

McCollum-Pratt Institute, The Johns Hopkins University, Baltimore, Maryland.

Bessman M J

References (13)

13 references, click to expand

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Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.90.3.724-728.1965

Published

1965-09-00

Pages

724-8

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC315717

External Links

PubMed source DOI: 10.1128/jb.90.3.724-728.1965 PMC Full Text View journal details

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Assay for the Killing Properties of T2 Bacteriophage and Their "Ghosts".

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