Amino Acid Analysis of the Isolated and Purified Components from Crystalline Toxin of Clostridium botulinum Type A.

Infection and immunity ·Vol. 2 ·No. 5 ·1970-11-00 ·Pages 679-80

Boroff DA, Meloche HP, Dasgupta BR

Abstract

The toxic and hemagglutinating components of the crystalline toxin of Clostridium botulinum were analyzed for their amino acid content and were proven to be completely different proteins.

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Boroff D A

Laboratory of Immunology, Research Laboratories, Albert Einstein Medical Center, Philadelphia, Pennsylvania 19141.

Meloche H P

Dasgupta B R

References (9)

9 references, click to expand

Chromatographic isolation of hemagglutinin-free neurotoxin from crystalline toxin of Clostridium botulinum type A.
Biochim Biophys Acta. 1967 Dec 12;147(3):603-5 PMID: 4866586
Separation of toxin and hemagglutinin from crystalline toxin of Clostridium botulinum type A by anion exchange chromatography and determination of their dimensions by gel filtration.
J Biol Chem. 1968 Mar 10;243(5):1065-72 PMID: 4867941
Ultracentrifugal analysis of the crystalline toxin and isolated fractions of Clostridium botulinum type A.
J Biol Chem. 1966 Nov 25;241(22):5165-7 PMID: 4959143
Cation-exchange chromatography of Clostridium botulinum type A toxin on amberlite IRC-50 resin at pH 5.55.
Biochim Biophys Acta. 1968 Dec 3;168(3):522-31 PMID: 4973552
Chromatographic fractionation of the crystalline toxin of Clostridium botulinum type A.
Biochem Biophys Res Commun. 1966 Mar 22;22(6):750-6 PMID: 5329438
Amino acid composition of crystalline botulinum toxin, type A.
J Bacteriol. 1967 Jul;94(1):277-8 PMID: 5338972
Low molecular weight forms of type A botulinum toxin. II. Action of pepsin on intact and dissociated toxin.
Arch Biochem Biophys. 1963 Mar;100:414-21 PMID: 13998203
The lack of identity between hemagglutinin and the toxin of type A botulinal organism.
J Bacteriol. 1951 Jun;61(6):751-2 PMID: 14850434
Protein measurement with the Folin phenol reagent.
J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713

Article Info

Journal

Infection and immunity

Abbr.

Infect Immun

ISSN

0019-9567

DOI

10.1128/iai.2.5.679-680.1970

Published

1970-11-00

Pages

679-80

Language

English

Region

United States

NLM ID

0246127

PMCID

PMC416070

External Links

PubMed source DOI: 10.1128/iai.2.5.679-680.1970 PMC Full Text View journal details

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Amino Acid Analysis of the Isolated and Purified Components from Crystalline Toxin of Clostridium botulinum Type A.

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