Purification and Characterization of Two Proteins from Culture Filtrates of Mycobacterium tuberculosis H(37)Ra Strain.

Infection and immunity ·Vol. 1 ·No. 2 ·1970-02-00 ·Pages 164-8

Daniel TM, Ferguson LE

Abstract

Two proteins have been purified from culture filtrates of Mycobacterium tuberculosis, H(37)Ra strain by a procedure combining gel filtration, diethylaminoethyl (DEAE)-cellulose chromatography, and zone electrophoresis. The two proteins are similar in molecular weight but differ slightly in charge. The faster migrating protein, designated a(1), is not antigenic. The slower migrating protein, designated a(2), is antigenic both with respect to antisera and as a skin-testing antigen.

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Daniel T M

Department of Medicine, Case Western Reserve University, School of Medicine, Cleveland, Ohio 44106.

Ferguson L E

References (13)

13 references, click to expand

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Article Info

Journal

Infection and immunity

Abbr.

Infect Immun

ISSN

0019-9567

DOI

10.1128/iai.1.2.164-168.1970

Published

1970-02-00

Pages

164-8

Language

English

Region

United States

NLM ID

0246127

PMCID

PMC415872

External Links

PubMed source DOI: 10.1128/iai.1.2.164-168.1970 PMC Full Text View journal details

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Purification and Characterization of Two Proteins from Culture Filtrates of Mycobacterium tuberculosis H(37)Ra Strain.

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