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PMID: 16556617 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Stem cell markers: ABCG2 and MCM2 expression in retinoblastoma.

The British journal of ophthalmology ·Vol. 90 ·No. 7 ·2006-07-00 ·Pages 889-93

Mohan A, Kandalam M, Ramkumar HL, Gopal L, Krishnakumar S

Abstract

The authors studied the expression of cancer stem cell surface marker, ABCG2, and neural stem cell marker, MCM2, in retinoblastoma and correlated clinicopathologically. Among 39 retinoblastomas, 18 tumours were not subjected to preoperative/postoperative chemotherapy, 15 tumours underwent postoperative chemotherapy, and six tumours had preoperative chemotherapy. There were 20 tumours with no invasion and 19 tumours with invasion of choroid/optic nerve. ABCG2 and MCM2 expression was studied by immunohistochemistry. ABCG2 was positive in six of six and MCM2 was positive in five of six tumours that had recurred in the orbit or metastasised. ABCG2 was positive in 15/19 tumours with invasion. MCM2 was positive in 16/19 tumours with invasion. Invasive tumours showed higher expression of ABCG2 (p < 0.01) and MCM2 (p < 0.01) proteins. There was no correlation with differentiation and laterality of the tumours. Non-neoplastic retina was positive for ABCG2 and MCM2. ABCG2 and MCM2 were expressed more in invasive tumours. Further studies are needed to understand the significance of ABCG2 and MCM2 expression in retinoblastoma.

MeSH Terms
ATP Binding Cassette Transporter, Subfamily G, Member 2 ATP-Binding Cassette Transporters/analysis Adolescent Adult Antineoplastic Agents/therapeutic use Biomarkers, Tumor/analysis Cell Cycle Proteins/analysis Child Child, Preschool Female Humans Immunohistochemistry/methods Infant Male Minichromosome Maintenance Complex Component 2 Neoplasm Invasiveness Neoplasm Proteins/analysis Nuclear Proteins/analysis Retinal Neoplasms/chemistry,drug therapy,pathology Retinoblastoma/chemistry,drug therapy,pathology Statistics, Nonparametric Stem Cells/metabolism
Chemicals
ABCG2 protein, human ATP Binding Cassette Transporter, Subfamily G, Member 2 ATP-Binding Cassette Transporters Antineoplastic Agents Biomarkers, Tumor Cell Cycle Proteins Neoplasm Proteins Nuclear Proteins MCM2 protein, human Minichromosome Maintenance Complex Component 2
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Mohan A
Department of Ocular Pathology, Vision Research Foundation, Sankara Nethralaya, Chennai - 600 006, Tamil Nadu, India.
Kandalam M
Ramkumar H L
Gopal L
Krishnakumar S
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Article Info
Journal
The British journal of ophthalmology
Abbr.
Br J Ophthalmol
ISSN
0007-1161
DOI
10.1136/bjo.2005.089219
Published
2006-07-00
Epub
2006-00-23
Pages
889-93
Language
English
Region
England
NLM ID
0421041
PMCID
PMC1857132
Subset
IM
External Links
PubMed source DOI: 10.1136/bjo.2005.089219 PMC Full Text View journal details
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