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PMID: 16554836 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Optimization of membrane protein overexpression and purification using GFP fusions.

Nature methods ·Vol. 3 ·No. 4 ·2006-04-00 ·Pages 303-13

Drew D, Lerch M, Kunji E, Slotboom DJ, de Gier JW

Abstract

暂无摘要

MeSH Terms
Escherichia coli Proteins/genetics,isolation & purification,metabolism Gene Expression Regulation, Bacterial Green Fluorescent Proteins/chemistry,genetics,metabolism Luminescent Agents/pharmacology Membrane Proteins/genetics,isolation & purification,metabolism Recombinant Fusion Proteins/genetics,metabolism
Chemicals
Escherichia coli Proteins Luminescent Agents Membrane Proteins Recombinant Fusion Proteins Green Fluorescent Proteins
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Drew David
Department of Biochemistry and Biophysics, Stockholm University, SE-106 91 Stockholm, Sweden.
Lerch Mirjam
Kunji Edmund
Slotboom Dirk-Jan
de Gier Jan-Willem
Article Info
Journal
Nature methods
Abbr.
Nat Methods
ISSN
1548-7091
DOI
10.1038/nmeth0406-303
Published
2006-04-00
Pages
303-13
Language
English
Region
United States
NLM ID
101215604
Subset
IM
Grants
Medical Research Council · MC_U105663139 · United Kingdom
External Links
PubMed source DOI: 10.1038/nmeth0406-303 View journal details
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