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PMID: 16552031 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Active cytotoxic necrotizing factor 1 associated with outer membrane vesicles from uropathogenic Escherichia coli.

Infection and immunity ·Vol. 74 ·No. 4 ·2006-04-00 ·Pages 2022-30

Kouokam JC, Wai SN, Fällman M, Dobrindt U, Hacker J, Uhlin BE

Abstract

Cytotoxic necrotizing factor type 1 (CNF1) is one of the virulence factors produced by uropathogenic Escherichia coli (UPEC). How this toxin is translocated from the bacterial cytoplasm to the surrounding environment is not well understood. Our data suggest that CNF1 may be regarded as a secreted protein, since it could be detected in culture supernatants. Furthermore, we found that CNF1 was tightly associated to outer membrane vesicles, suggesting that such vesicles play a role in the secretion of this protein. Interestingly, vesicle samples containing CNF1 could exert the effects known for this protein on HeLa cell cultures, showing that CNF1 is transported by vesicles in its active form. Taken together, our results strongly suggest that outer membrane vesicles could be a means for the bacteria to deliver CNF1 to the environment and to the infected tissue. In addition, our results indicate that the histone-like nucleoid structuring protein H-NS has a role in the downregulation of CNF1 production and that it affects the outer membrane vesicle release in UPEC strain J96.

MeSH Terms
Bacterial Outer Membrane Proteins/genetics,metabolism Bacterial Toxins/genetics,metabolism Biological Transport, Active Blotting, Western Cytotoxins/metabolism DNA-Binding Proteins/physiology Escherichia coli/genetics,metabolism,pathogenicity,ultrastructure Escherichia coli Proteins/genetics,metabolism,physiology HeLa Cells Humans Mutation Transport Vesicles/metabolism
Chemicals
Bacterial Outer Membrane Proteins Bacterial Toxins Cytotoxins DNA-Binding Proteins Escherichia coli Proteins hns protein, E coli cytotoxic necrotizing factor type 1
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Kouokam J Clavin
Department of Molecular Biology, Umeå University, S-901 87 Umeå, Sweden.
Wai Sun Nyunt
Fällman Maria
Dobrindt Ulrich
Hacker Jörg
Uhlin Bernt Eric
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/IAI.74.4.2022-2030.2006
Published
2006-04-00
Pages
2022-30
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC1418910
Subset
IM
External Links
PubMed source DOI: 10.1128/IAI.74.4.2022-2030.2006 PMC Full Text View journal details
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