Abstract
An approach involving retroviral-mediated gene therapy for the treatment of neoplastic disease is described. This therapeutic approach is called "virus-directed enzyme/prodrug therapy" (VDEPT). The VDEPT approach exploits the transcriptional differences between normal and neoplastic cells to achieve selective killing of neoplastic cells. We now describe development of the VDEPT approach for the treatment of hepatocellular carcinoma. Replication-defective, amphotrophic retroviruses were constructed containing a chimeric varicella-zoster virus thymidine kinase (VZV TK) gene that is transcriptionally regulated by either the hepatoma-associated alpha-fetoprotein or liver-associated albumin transcriptional regulatory sequences. Subsequent to retroviral infection, expression of VZV TK was limited to either alpha-fetoprotein- or albumin-positive cells, respectively. VZV TK metabolically activated the nontoxic prodrug 6-methoxypurine arabinonucleoside (araM), ultimately leading to the formation of the cytotoxic anabolite adenine arabinonucleoside triphosphate (araATP). Cells that selectively expressed VZV TK became selectively sensitive to araM due to the VZV TK-dependent anabolism of araM to araATP. Hence, these retroviral-delivered chimeric genes generated tissue-specific expression of VZV TK, tissue-specific anabolism of araM to araATP, and tissue-specific cytotoxicity due to araM exposure. By utilizing such retroviral vectors, araM was anabolized to araATP in hepatoma cells, producing a selective cytotoxic effect.
MeSH Terms
Animals
Arabinonucleosides/administration & dosage
Base Sequence
Carcinoma, Hepatocellular/therapy
Cloning, Molecular
Gene Expression
Genetic Therapy
Genetic Vectors
Growth Inhibitors/administration & dosage
Herpesvirus 3, Human/genetics
Humans
In Vitro Techniques
Liver Neoplasms/therapy
Molecular Sequence Data
Oligonucleotides/chemistry
Prodrugs/administration & dosage
Rats
Thymidine Kinase/administration & dosage,genetics
Tumor Cells, Cultured
Chemicals
Arabinonucleosides
Growth Inhibitors
Oligonucleotides
Prodrugs
6-methoxypurine arabinoside
Thymidine Kinase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Huber B E
Division of Experimental Therapy, Wellcome Research Laboratories, Research Triangle Park, NC 27709.
Richards C A
Krenitsky T A
References (17)
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