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PMID: 16537443 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

High-amylose wheat generated by RNA interference improves indices of large-bowel health in rats.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 103 ·No. 10 ·2006-03-07 ·Pages 3546-51

Regina A, Bird A, Topping D, Bowden S, Freeman J, Barsby T, Kosar-Hashemi B, Li Z, Rahman S, Morell M

Abstract

Foods high in resistant starch have the potential to improve human health and lower the risk of serious noninfectious diseases. RNA interference was used to down-regulate the two different isoforms of starch-branching enzyme (SBE) II (SBEIIa and SBEIIb) in wheat endosperm to raise its amylose content. Suppression of SBEIIb expression alone had no effect on amylose content; however, suppression of both SBEIIa and SBEIIb expression resulted in starch containing >70% amylose. When the >70% amylose wheat grain was fed to rats in a diet as a wholemeal, several indices of large-bowel function, including short-chain fatty acids, were improved relative to standard wholemeal wheat. These results indicate that this high-amylose wheat has a significant potential to improve human health through its resistant starch content.

MeSH Terms
1,4-alpha-Glucan Branching Enzyme/antagonists & inhibitors,genetics,metabolism Amylose/metabolism Animals Diet Food, Genetically Modified Genetic Engineering Glucans/chemistry Health Status Humans Intestine, Large/metabolism Isoenzymes/antagonists & inhibitors,genetics,metabolism Male Plants, Genetically Modified RNA Interference Rats Rats, Sprague-Dawley Starch/chemistry,metabolism Triticum/genetics,metabolism
Chemicals
Glucans Isoenzymes Starch Amylose 1,4-alpha-Glucan Branching Enzyme
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Regina Ahmed
Commonwealth Scientific and Industrial Research Organization, Food Futures National Research Flagship, P.O. Box 93, North Ryde 1670, NSW, Australia.
Bird Anthony
Topping David
Bowden Sarah
Freeman Judy
Barsby Tina
Kosar-Hashemi Behjat
Li Zhongyi
Rahman Sadequr
Morell Matthew
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.0510737103
Published
2006-03-07
Epub
2006-00-28
Pages
3546-51
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC1450120
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.0510737103 PMC Full Text View journal details
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