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PMID: 16537418 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Suppressing Brownian motion of individual biomolecules in solution.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 103 ·No. 12 ·2006-03-21 ·Pages 4362-5

Cohen AE, Moerner WE

Abstract

Single biomolecules in free solution have long been of interest for detailed study by optical methods, but Brownian motion prevents the observation of one single molecule for extended periods. We have used an anti-Brownian electrokinetic (ABEL) trap to trap individual protein molecules in free solution, under ambient conditions, without requiring any attachment to beads or surfaces. We also demonstrate trapping and manipulation of single virus particles, lipid vesicles, and fluorescent semiconductor nanocrystals.

MeSH Terms

Fluorescent Dyes/chemistry Microspheres Models, Chemical Motion Optics and Photonics Solutions Tobacco Mosaic Virus/chemistry Virion/chemistry

Chemicals

Fluorescent Dyes Solutions

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Cohen Adam E

Department of Physics, Stanford University, 381 Via Pueblo Mall, Stanford, CA 94305, USA. aecohen@stanford.edu

Moerner W E

References (12)

12 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.0509976103

aecohen@stanford.edu

Published

2006-03-21

Epub

2006-00-14

Pages

4362-5

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC1450176

Subset

External Links

PubMed source DOI: 10.1073/pnas.0509976103 PMC Full Text View journal details

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