A simple, efficient method for the separation of humic substances and DNA from environmental samples.

Applied and environmental microbiology ·Vol. 63 ·No. 12 ·1997-12-00 ·Pages 4993-5

Jackson CR, Harper JP, Willoughby D, Roden EE, Churchill PF

Abstract

Three different gels (Sepharose 4B, Sephadex G-200, and Sephadex G-50) were evaluated as a means of removing humic contaminants from DNA extracts of environmental samples. Sepharose 4B gave superior separation of DNA from humics, and DNA purified in this way showed consistently greater amplification than DNA purified by the other materials.

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Jackson C R

Harper J P

Willoughby D

Roden E E

Churchill P F

References (18)

18 references, click to expand

Molecular microbial diversity of an agricultural soil in Wisconsin.
Appl Environ Microbiol. 1996 Jun;62(6):1935-43 PMID: 8787391
Automated polymerase chain reaction in capillary tubes with hot air.
Nucleic Acids Res. 1989 Jun 12;17(11):4353-7 PMID: 2740218
Analysis of ammonia-oxidizing bacteria of the beta subdivision of the class Proteobacteria in coastal sand dunes by denaturing gradient gel electrophoresis and sequencing of PCR-amplified 16S ribosomal DNA fragments.
Appl Environ Microbiol. 1997 Apr;63(4):1489-97 PMID: 9097446
Direct extraction and purification of rRNA for ecological studies.
Appl Environ Microbiol. 1993 Mar;59(3):915-8 PMID: 7683186
Detection of low numbers of bacterial cells in soils and sediments by polymerase chain reaction.
Appl Environ Microbiol. 1992 Feb;58(2):754-7 PMID: 1610201
Remarkable archaeal diversity detected in a Yellowstone National Park hot spring environment.
Proc Natl Acad Sci U S A. 1994 Mar 1;91(5):1609-13 PMID: 7510403
DNA Probe Method for the Detection of Specific Microorganisms in the Soil Bacterial Community.
Appl Environ Microbiol. 1988 Mar;54(3):703-711 PMID: 16347582
DNA amplification to enhance detection of genetically engineered bacteria in environmental samples.
Appl Environ Microbiol. 1988 Sep;54(9):2185-91 PMID: 3190225
'Touchdown' PCR to circumvent spurious priming during gene amplification.
Nucleic Acids Res. 1991 Jul 25;19(14):4008 PMID: 1861999
Rapid method for separation of bacterial DNA from humic substances in sediments for polymerase chain reaction.
Appl Environ Microbiol. 1992 Jul;58(7):2292-5 PMID: 1386212
Minimizing the time required for DNA amplification by efficient heat transfer to small samples.
Anal Biochem. 1990 May 1;186(2):328-31 PMID: 2363506
Rapid method for direct extraction of DNA from soil and sediments.
Appl Environ Microbiol. 1991 Apr;57(4):1070-4 PMID: 1647749
Polymerase chain reaction amplification of naphthalene-catabolic and 16S rRNA gene sequences from indigenous sediment bacteria.
Appl Environ Microbiol. 1993 Mar;59(3):687-94 PMID: 7683182
DNA recovery from soils of diverse composition.
Appl Environ Microbiol. 1996 Feb;62(2):316-22 PMID: 8593035
Polyvinylpyrrolidone-agarose gel electrophoresis purification of polymerase chain reaction-amplifiable DNA from soils.
Appl Environ Microbiol. 1993 Jun;59(6):1972-4 PMID: 16348974
Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA.
Appl Environ Microbiol. 1993 Mar;59(3):695-700 PMID: 7683183
Recovery of DNA from soils and sediments.
Appl Environ Microbiol. 1988 Dec;54(12):2908-15 PMID: 2851961
Rapid cycle DNA amplification: time and temperature optimization.
Biotechniques. 1991 Jan;10(1):76-83 PMID: 2003928

Article Info

Journal

Applied and environmental microbiology

Abbr.

Appl Environ Microbiol

ISSN

0099-2240

DOI

10.1128/aem.63.12.4993-4995.1997

Published

1997-12-00

Pages

4993-5

Language

English

Region

United States

NLM ID

7605801

PMCID

PMC1389316

External Links

PubMed source DOI: 10.1128/aem.63.12.4993-4995.1997 PMC Full Text View journal details

Analysis Services

A simple, efficient method for the separation of humic substances and DNA from environmental samples.

Contact

WeChat Official Account