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PMID: 16535431 Published · ppublish English Journal Article

Rapid Extraction of DNA and rRNA from Sediments by a Novel Hydroxyapatite Spin-Column Method.

Applied and environmental microbiology ·Vol. 62 ·No. 10 ·1996-10-00 ·Pages 3905-7

Purdy KJ, Embley TM, Takii S, Nedwell DB

Abstract

We describe a novel hydroxyapatite spin-column method of nucleic acid extraction from natural sediments by which DNA and rRNA can be extracted separately. This very rapid method produces pure nucleic acid that can be utilized in some of the most common molecular biological procedures used in the analysis of natural microbial communities.

Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Purdy K J
Embley T M
Takii S
Nedwell D B
References (12)
12 references, click to expand
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Article Info
Journal
Applied and environmental microbiology
Abbr.
Appl Environ Microbiol
ISSN
0099-2240
DOI
10.1128/aem.62.10.3905-3907.1996
Published
1996-10-00
Pages
3905-7
Language
English
Region
United States
NLM ID
7605801
PMCID
PMC1388969
External Links
PubMed source DOI: 10.1128/aem.62.10.3905-3907.1996 PMC Full Text View journal details
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