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PMID: 16533168 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Control by cytochrome c oxidase of the cellular oxidative phosphorylation system depends on the mitochondrial energy state.

The Biochemical journal ·Vol. 396 ·No. 3 ·2006-06-15 ·Pages 573-83

Piccoli C, Scrima R, Boffoli D, Capitanio N

Abstract

Recent measurements of the flux control exerted by cytochrome c oxidase on the respiratory activity in intact cells have led to a re-appraisal of its regulatory function. We have further extended this in vivo study in the framework of the Metabolic Control Analysis and evaluated the impact of the mitochondrial transmembrane electrochemical potential (Deltamu(H+)) on the control strength of the oxidase. The results indicate that, under conditions mimicking the mitochondrial State 4 of respiration, both the flux control coefficient and the threshold value of cytochrome oxidase are modified with respect to the uncoupled condition. The results obtained are consistent with a model based on changes in the assembly state of the oxidative phosphorylation enzyme complexes and possible implications in the understanding of exercise-intolerance of human neuromuscular degenerative diseases are discussed.

MeSH Terms
Ascorbic Acid/metabolism Cell Line, Tumor Electron Transport Complex IV/metabolism Energy Metabolism/physiology Humans Kinetics Membrane Potentials/physiology Mitochondria/physiology Mitochondrial Membranes/physiology Oligomycins/pharmacology Oxidative Phosphorylation/drug effects Oxygen Consumption Tetramethylphenylenediamine/pharmacology Valinomycin/pharmacology
Chemicals
Oligomycins Valinomycin Electron Transport Complex IV Tetramethylphenylenediamine Ascorbic Acid
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Piccoli Claudia
Department of Biomedical Science, University of Foggia, 71100 Foggia, Italy.
Scrima Rosella
Boffoli Domenico
Capitanio Nazzareno
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
1470-8728
DOI
10.1042/BJ20060077
Published
2006-06-15
Pages
573-83
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1482809
Subset
IM
External Links
PubMed source DOI: 10.1042/BJ20060077 PMC Full Text View journal details
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