Home LiteratureArticle Details
PMID: 1651410 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Mouse DNA primase plays the principal role in determination of permissiveness for polyomavirus DNA replication.

Journal of virology ·Vol. 65 ·No. 9 ·1991-09-00 ·Pages 4874-81

Eki T, Enomoto T, Masutani C, Miyajima A, Takada R, Murakami Y, Ohno T, Hanaoka F, Ui M

Abstract

We have investigated the species-specific replication of polyomavirus DNA in the cell-free system that was established previously (Y. Murakami, T. Eki, M. Yamada, C. Prives, and J. Hurwitz, Proc. Natl. Acad. Sci. USA 83:6347-6351, 1986). Extracts from various species of cells supported polyomavirus DNA replication in a species-specific manner that was consistent with the host range specificity of polyomavirus; extracts prepared from mouse and hamster cells were active, whereas extracts prepared from human, monkey, and insect cells were inactive. The addition of DNA polymerase alpha-primase purified from mouse cells induced the replication of polyomavirus DNA in a cell-free system containing polyomavirus large tumor antigen and nonpermissive cell extracts, such as human and insect cell extracts. Isolated mouse DNA primase alone also induced polyomavirus DNA replication in human cell extracts but not in insect cell extracts, indicating that mouse DNA primase plays the principal role in determining permissiveness for polyomavirus DNA replication.

MeSH Terms
Animals Antigens, Polyomavirus Transforming/physiology Cell-Free System Cells, Cultured DNA Polymerase II/metabolism DNA Primase DNA Replication DNA, Viral/biosynthesis HeLa Cells Humans In Vitro Techniques Mice Polyomavirus/genetics,growth & development RNA Nucleotidyltransferases/physiology Simian virus 40/genetics,growth & development Species Specificity Virus Replication
Chemicals
Antigens, Polyomavirus Transforming DNA, Viral DNA Primase RNA Nucleotidyltransferases DNA Polymerase II
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Eki T
Department of Physiological Chemistry, Faculty of Pharmaceutical Sciences, University of Tokyo, Japan.
Enomoto T
Masutani C
Miyajima A
Takada R
Murakami Y
Ohno T
Hanaoka F
Ui M
References (29)
29 references, click to expand
  1. Enzymatic synthesis of deoxyribonucleic acid. IX. The polymerase formed after T2 bacteriophage infection of Escherichia coli: a new enzyme.
    J Biol Chem. 1962 Feb;237:519-25 PMID: 13861902
  2. Establishment of cell lines in vitro from a mammary ascites tumor of mouse and biological properties of the established lines in a serum containing medium.
    Tohoku J Exp Med. 1966 Jan 25;88(1):69-84 PMID: 5920633
  3. The in vitro replication of DNA containing the SV40 origin.
    J Biol Chem. 1990 Oct 25;265(30):18043-6 PMID: 1976634
  4. SV40 T antigen binds directly to the large subunit of purified DNA polymerase alpha.
    EMBO J. 1990 Oct;9(10):3329-36 PMID: 1698613
  5. In vitro replication of DNA containing either the SV40 or the polyoma origin.
    Philos Trans R Soc Lond B Biol Sci. 1987 Dec 15;317(1187):439-53 PMID: 2894681
  6. DNA primase-DNA polymerase alpha assembly from mouse FM3A cells. Purification of constituting enzymes, reconstitution, and analysis of RNA priming as coupled to DNA synthesis.
    J Biol Chem. 1989 Jun 15;264(17):10065-71 PMID: 2722861
  7. Species-specific in vitro synthesis of DNA containing the polyoma virus origin of replication.
    Proc Natl Acad Sci U S A. 1986 Sep;83(17):6347-51 PMID: 3018726
  8. Characterization of DNA polymerase alpha activity from a mouse DNA temperature-sensitive mutant, strain tsFT20, which shows a defect in DNA polymerase alpha activity at restrictive temperatures.
    Arch Biochem Biophys. 1988 Feb 1;260(2):552-60 PMID: 3341757
  9. Role of DNA polymerase alpha and DNA primase in simian virus 40 DNA replication in vitro.
    Proc Natl Acad Sci U S A. 1986 May;83(9):2869-73 PMID: 3010320
  10. Mouse p53 inhibits SV40 origin-dependent DNA replication.
    Nature. 1987 Oct 1-7;329(6138):458-60 PMID: 2821401
  11. SV40 DNA replication.
    J Biol Chem. 1988 Dec 5;263(34):17889-92 PMID: 2848017
  12. Common regulatory elements control gene expression from polyoma early and late promoters in cells transformed by chimeric plasmids.
    Mol Cell Biol. 1985 Aug;5(8):2070-9 PMID: 3018549
  13. Replication and supercoiling of simian virus 40 DNA in cell extracts from human cells.
    Mol Cell Biol. 1985 Aug;5(8):2051-60 PMID: 3018548
  14. T-antigen-DNA polymerase alpha complex implicated in simian virus 40 DNA replication.
    Mol Cell Biol. 1986 Nov;6(11):4077-87 PMID: 3025630
  15. Simian virus 40 DNA replication in vitro: specificity of initiation and evidence for bidirectional replication.
    Mol Cell Biol. 1985 Jun;5(6):1238-46 PMID: 2993858
  16. In vitro replication of duplex circular DNA containing the simian virus 40 DNA origin site.
    Proc Natl Acad Sci U S A. 1985 Sep;82(17):5710-4 PMID: 2994044
  17. Studies of the DNA helicase-RNA primase unit from bacteriophage T4. A trinucleotide sequence on the DNA template starts RNA primer synthesis.
    J Biol Chem. 1986 May 25;261(15):7001-10 PMID: 2422175
  18. The murine p53 protein blocks replication of SV40 DNA in vitro by inhibiting the initiation functions of SV40 large T antigen.
    Cell. 1989 May 5;57(3):379-92 PMID: 2541911
  19. Herpes simplex virus 1 helicase-primase: a complex of three herpes-encoded gene products.
    Proc Natl Acad Sci U S A. 1989 Apr;86(7):2186-9 PMID: 2538835
  20. Simian virus 40 DNA replication in vitro.
    Proc Natl Acad Sci U S A. 1984 Nov;81(22):6973-7 PMID: 6095264
  21. Purification and characterization of two forms of DNA polymerase alpha from mouse FM3A cells: a DNA polymerase alpha-primase complex and a free DNA polymerase alpha.
    Cell Struct Funct. 1985 Jun;10(2):161-71 PMID: 4005961
  22. Peptide mapping of the four subunits of the mouse DNA polymerase alpha-primase complex.
    Biochem Biophys Res Commun. 1990 Jul 31;170(2):589-95 PMID: 2383257
  23. DNA helicase and nucleoside-5'-triphosphatase activities of polyoma virus large tumor antigen.
    Biochemistry. 1990 Jan 30;29(4):1003-9 PMID: 2160269
  24. Primate's p53 inhibits SV40 DNA replication in vitro.
    Biochem Biophys Res Commun. 1990 Apr 30;168(2):604-8 PMID: 2159296
  25. Monoclonal antibodies specific for simian virus 40 tumor antigens.
    J Virol. 1981 Sep;39(3):861-9 PMID: 6169844
  26. Polyomavirus: an overview of its unique properties.
    Adv Cancer Res. 1983;39:183-268 PMID: 6312779
  27. Initiation of simian virus 40 DNA replication in vitro.
    J Virol. 1983 Nov;48(2):481-91 PMID: 6312104
  28. Production of SV40 virus in heterokaryons of transformed and susceptible cells.
    Proc Natl Acad Sci U S A. 1967 Oct;58(4):1396-403 PMID: 4295828
  29. Studies on the initiation and elongation reactions in the simian virus 40 DNA replication system.
    Proc Natl Acad Sci U S A. 1990 Dec;87(24):9712-6 PMID: 2175912
Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.65.9.4874-4881.1991
Published
1991-09-00
Pages
4874-81
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC248947
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.65.9.4874-4881.1991 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2