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PMID: 16513846 Published · epublish English Journal Article Research Support, Non-U.S. Gov't

Nuclear import of the transcription factor SHOOT MERISTEMLESS depends on heterodimerization with BLH proteins expressed in discrete sub-domains of the shoot apical meristem of Arabidopsis thaliana.

Nucleic acids research ·Vol. 34 ·No. 4 ·2006-00-00 ·Pages 1281-92

Cole M, Nolte C, Werr W

Abstract

The gene SHOOT MERISTEMLESS (STM) is required for the initiation and the maintenance of the shoot apical meristem (SAM) in Arabidopsis and encodes a MEINOX/three amino acid loop extension (TALE)-HD-type transcription factor. Translational fusions with the green fluorescent protein showed that STM is not nuclear by default. In a yeast two-hybrid screen performed with a meristem-enriched cDNA library, three interacting BLH (Bel1-like homeodomain) transcription factors were identified. According to bimolecular fluorescence complementation, STM is targeted into the nuclear compartment through heterodimerization with BLH partner proteins, which are expressed in distinct SAM domains from the center to the periphery. On a functional level, overexpression experiments in transgenic Arabidopsis plants suggest that individual heterodimers provide distinct contributions. These results contribute to our understanding of the STM transcription factor function in the SAM and also shed new light on the evolution of the TALE-HD super gene family in animal and plant lineages.

MeSH Terms
Active Transport, Cell Nucleus Arabidopsis/anatomy & histology,genetics,metabolism Arabidopsis Proteins/genetics,metabolism Cell Nucleus/metabolism Dimerization Homeodomain Proteins/genetics,metabolism Immunoprecipitation Meristem/genetics,metabolism Plants, Genetically Modified/metabolism Protein Structure, Tertiary RNA, Plant/analysis Transcription Factors/chemistry,genetics,metabolism
Chemicals
Arabidopsis Proteins Homeodomain Proteins RNA, Plant STM protein, Arabidopsis Transcription Factors
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Cole Melanie
Institut für Entwicklungsbiologie, Gyrhofstr. 17, D-50923 Köln, Germany.
Nolte Carolin
Werr Wolfgang
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
1362-4962
DOI
10.1093/nar/gkl016
Published
2006-00-00
Epub
2006-00-02
Pages
1281-92
Language
English
Region
England
NLM ID
0411011
PMCID
PMC1388269
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/gkl016 PMC Full Text View journal details
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