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PMID: 16508010 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, U.S. Gov't, Non-P.H.S.

Nipped-A, the Tra1/TRRAP subunit of the Drosophila SAGA and Tip60 complexes, has multiple roles in Notch signaling during wing development.

Molecular and cellular biology ·Vol. 26 ·No. 6 ·2006-03-00 ·Pages 2347-59

Gause M, Eissenberg JC, Macrae AF, Dorsett M, Misulovin Z, Dorsett D

Abstract

The Notch receptor controls development by activating transcription of specific target genes in response to extracellular signals. The factors that control assembly of the Notch activator complex on target genes and its ability to activate transcription are not fully known. Here we show, through genetic and molecular analysis, that the Drosophila Nipped-A protein is required for activity of Notch and its coactivator protein, mastermind, during wing development. Nipped-A and mastermind also colocalize extensively on salivary gland polytene chromosomes, and reducing Nipped-A activity decreases mastermind binding. Nipped-A is the fly homologue of the yeast Tra1 and human TRRAP proteins and is a key component of both the SAGA and Tip60 (NuA4) chromatin-modifying complexes. We find that, like Nipped-A, the Ada2b component of SAGA and the domino subunit of Tip60 are also required for mastermind function during wing development. Based on these results, we propose that Nipped-A, through the action of the SAGA and Tip60 complexes, facilitates assembly of the Notch activator complex and target gene transcription.

MeSH Terms
Adaptor Proteins, Signal Transducing Animals Drosophila Proteins/genetics,metabolism Drosophila melanogaster/embryology,growth & development Embryo, Nonmammalian Female Gene Expression Regulation, Developmental Histone Acetyltransferases/genetics,metabolism Male Molecular Sequence Data Multiprotein Complexes/metabolism Mutation Nuclear Proteins/genetics,metabolism Protein Subunits Receptors, Notch/genetics,metabolism Signal Transduction Transcription Factors/metabolism Veins/growth & development Wings, Animal/growth & development
Chemicals
Ada2a protein, Drosophila Adaptor Proteins, Signal Transducing Drosophila Proteins Multiprotein Complexes N protein, Drosophila NIPPED-A protein, Drosophila Nuclear Proteins Protein Subunits Receptors, Notch Transcription Factors mam protein, Drosophila transformation-transcription domain-associated protein Histone Acetyltransferases Tip60 protein, Drosophila
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Gause Maria
Edward A. Doisy Department of Biochemistry and Molecular Biology, Saint Louis University School of Medicine, 1402 South Grand Boulevard, Saint Louis, MO 63104, USA.
Eissenberg Joel C
Macrae Amy F
Dorsett Maia
Misulovin Ziva
Dorsett Dale
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.26.6.2347-2359.2006
Published
2006-03-00
Pages
2347-59
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC1430305
Subset
IM
Grants
NIGMS NIH HHS · R01 GM055683 · United States
NIGMS NIH HHS · R01 GM055683-05S1 · United States
NIGMS NIH HHS · R01 GM055683-06A1 · United States
NIGMS NIH HHS · GM055683 · United States
NIGMS NIH HHS · R01 GM055683-07 · United States
NIGMS NIH HHS · R01 GM055683-08 · United States
Databases
GENBANK
DQ352451
External Links
PubMed source DOI: 10.1128/MCB.26.6.2347-2359.2006 PMC Full Text View journal details
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