Home LiteratureArticle Details
PMID: 1648416 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Voltage-dependent gap junction channels are formed by connexin32, the major gap junction protein of rat liver.

Biophysical journal ·Vol. 59 ·No. 4 ·1991-04-00 ·Pages 920-5

Moreno AP, de Carvalho AC, Verselis V, Eghbali B, Spray DC

Abstract

We report here experiments undertaken in pairs of hepatocytes that demonstrate a marked voltage sensivity of junctional conductance and, thus, contradict earlier findings reported by this laboratory (Spray, D.C., R.D.ginzberg, E.A., E. A. Morales, Z. Gatmaitan and I.M. Arias, 1986, J. Cell Biol. 101:135-144; Spray C.D. R.L. White, A.C. Campos de Carvalho, and M.V.L. Bennett. 1984. Biophys. J. 45:219-230) and by others (Dahl, G., T. Moller, D. Paul, R. Voellmy, and R. Werner. 1987. Science [Wash. DC] 236:1290-1293; Riverdin, E.C., and R. Weingart. 1988. Am. J. Physiol. 254:C226-C234). Expression in exogenous systems, lipid bilayers in which fragments of isolated gap junction membranes were incorporated (Young, J.D.-E., Z. Cohn, and N.B. Gilula. 1987. Cell. 48:733-743.) and noncommunicating cells transfected with connexin32 cDNA (Eghbali, B., J.A. Kessler, and D.C. Spray. 1990. Proc. Natl. Acad. Sci. USA. 87:1328-1331), support these findings and indicate that the voltage-dependent channel is composed of connexin32, the major gap junction protein of rat liver (Paul, D. 1986. J. Cell Biol. 103:123-134).

MeSH Terms
Animals Carcinoma, Hepatocellular Cell Line Cells, Cultured Connexins Electric Conductivity Humans Intercellular Junctions/physiology Lipid Bilayers Liver/physiology Liver Neoplasms Membrane Proteins/genetics,physiology Rats Transfection
Chemicals
Connexins Lipid Bilayers Membrane Proteins
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Moreno A P
Department of Neuroscience, Albert Einstein College of Medicine, Bronx, New York 10461.
de Carvalho A C
Verselis V
Eghbali B
Spray D C
References (35)
35 references, click to expand
  1. Transmission at the giant motor synapses of the crayfish.
    J Physiol. 1959 Mar 3;145(2):289-325 PMID: 13642302
  2. Kinetics of the opening and closing of individual excitability-inducing material channels in a lipid bilayer.
    J Gen Physiol. 1974 Jun;63(6):707-21 PMID: 4829526
  3. Biophysical properties of gap junctions between freshly dispersed pairs of mouse pancreatic beta cells.
    Biophys J. 1991 Jan;59(1):76-92 PMID: 2015391
  4. The mechanism of rectification at the electrotonic motor giant synapse of the crayfish.
    Nature. 1986 Sep 4-10;323(6083):63-5 PMID: 3748182
  5. Single-channel events and gating behavior of the cardiac gap junction channel.
    Proc Natl Acad Sci U S A. 1988 May;85(10):3431-4 PMID: 2453059
  6. Electrophysiological properties of gap junctions between dissociated pairs of rat hepatocytes.
    J Cell Biol. 1986 Jul;103(1):135-44 PMID: 3722262
  7. Cloning and characterization of human and rat liver cDNAs coding for a gap junction protein.
    J Cell Biol. 1986 Sep;103(3):767-76 PMID: 2875078
  8. Physiology and pharmacology of gap junctions.
    Annu Rev Physiol. 1985;47:281-303 PMID: 2859833
  9. Connexin43: a protein from rat heart homologous to a gap junction protein from liver.
    J Cell Biol. 1987 Dec;105(6 Pt 1):2621-9 PMID: 2826492
  10. Molecular cloning of cDNA for rat liver gap junction protein.
    J Cell Biol. 1986 Jul;103(1):123-34 PMID: 3013898
  11. Expression of functional cell-cell channels from cloned rat liver gap junction complementary DNA.
    Science. 1987 Jun 5;236(4806):1290-3 PMID: 3035715
  12. Formation of gap junctions by expression of connexins in Xenopus oocyte pairs.
    Cell. 1989 Apr 7;57(1):145-55 PMID: 2467743
  13. Properties of single gap junctional channels between isolated neonatal rat heart cells.
    Am J Physiol. 1988 Oct;255(4 Pt 2):H770-82 PMID: 2459974
  14. Isolated liver gap junctions: gating of transjunctional currents is similar to that in intact pairs of rat hepatocytes.
    Proc Natl Acad Sci U S A. 1986 Aug;83(15):5494-7 PMID: 2426699
  15. Single-channel currents of an intercellular junction.
    Nature. 1985 Sep 26-Oct 2;317(6035):331-5 PMID: 2413362
  16. Differential expression of three gap junction proteins in developing and mature brain tissues.
    Proc Natl Acad Sci U S A. 1989 Dec;86(24):10148-52 PMID: 2557621
  17. Sequence and tissue distribution of a second protein of hepatic gap junctions, Cx26, as deduced from its cDNA.
    J Cell Biol. 1989 Dec;109(6 Pt 2):3391-401 PMID: 2557354
  18. cAMP delays disappearance of gap junctions between pairs of rat hepatocytes in primary culture.
    Am J Physiol. 1989 Jul;257(1 Pt 1):C1-11 PMID: 2546432
  19. Comparative characterization of the 21-kD and 26-kD gap junction proteins in murine liver and cultured hepatocytes.
    J Cell Biol. 1989 Mar;108(3):1039-51 PMID: 2537831
  20. Electrical properties of the gap junctional membrane studied in rat liver cell pairs.
    Am J Physiol. 1988 Feb;254(2 Pt 1):C226-34 PMID: 3348363
  21. Functional assembly of gap junction conductance in lipid bilayers: demonstration that the major 27 kd protein forms the junctional channel.
    Cell. 1987 Mar 13;48(5):733-43 PMID: 3815522
  22. Differential regulation of the levels of three gap junction mRNAs in Xenopus embryos.
    J Cell Biol. 1990 Mar;110(3):597-605 PMID: 2155241
  23. Expression of gap junction channels in communication-incompetent cells after stable transfection with cDNA encoding connexin 32.
    Proc Natl Acad Sci U S A. 1990 Feb;87(4):1328-31 PMID: 2154741
  24. Voltage-dependent gating of gap junction channels in embryonic chick ventricular cell pairs.
    Am J Physiol. 1990 Apr;258(4 Pt 1):C662-72 PMID: 2333952
  25. Gating of gap junction channels.
    Biophys J. 1984 Jan;45(1):219-30 PMID: 6324905
  26. Control of intercellular communication by voltage dependence of gap junctional conductance.
    J Neurosci. 1983 Jan;3(1):79-100 PMID: 6822860
  27. Bidirectional transmission at the rectifying electrotonic synapse: a voltage-dependent process.
    Science. 1983 Apr 1;220(4592):84-7 PMID: 6298940
  28. Cell-to-cell channels with two independently regulated gates in series: analysis of junctional conductance modulation by membrane potential, calcium, and pH.
    J Membr Biol. 1983;73(1):69-89 PMID: 6306241
  29. Kinetic properties of a voltage-dependent junctional conductance.
    J Gen Physiol. 1981 Jan;77(1):95-117 PMID: 6259275
  30. Gap junctional conductance is a simple and sensitive function of intracellular pH.
    Science. 1981 Feb 13;211(4483):712-5 PMID: 6779379
  31. Voltage dependence of junctional conductance in early amphibian embryos.
    Science. 1979 Apr 27;204(4391):432-4 PMID: 312530
  32. Monosynaptic chemical and electrical connexions between sensory and motor cells in the central nervous system of the leech.
    J Physiol. 1970 Aug;209(3):647-67 PMID: 5499801
  33. Localization and electrical characteristics of a giant synapse in the spinal cord of the lamprey.
    J Physiol. 1975 Oct;251(2):395-407 PMID: 1185671
  34. A rectifying electrotonic synapse in the central nervous system of a vertebrate.
    J Gen Physiol. 1969 Feb;53(2):211-37 PMID: 4303657
  35. A quantitative description of membrane current and its application to conduction and excitation in nerve.
    J Physiol. 1952 Aug;117(4):500-44 PMID: 12991237
Article Info
Journal
Biophysical journal
Abbr.
Biophys J
ISSN
0006-3495
DOI
10.1016/S0006-3495(91)82305-0
Published
1991-04-00
Pages
920-5
Language
English
Region
United States
NLM ID
0370626
PMCID
PMC1281258
Subset
IM
Grants
NHLBI NIH HHS · HL 34479 · United States
NINDS NIH HHS · NS 07512 · United States
NINDS NIH HHS · NS 16524 · United States
External Links
PubMed source DOI: 10.1016/S0006-3495(91)82305-0 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2