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PMID: 16482217 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Structural basis for the inhibition of activin signalling by follistatin.

The EMBO journal ·Vol. 25 ·No. 5 ·2006-03-08 ·Pages 1035-45

Harrington AE, Morris-Triggs SA, Ruotolo BT, Robinson CV, Ohnuma S, Hyvönen M

Abstract

The secreted, multidomain protein follistatin binds activins with high affinity, inhibiting their receptor interaction. We have dissected follistatin's domain structure and shown that the minimal activin-inhibiting fragment of follistatin is comprised of the first and second Fs domains (Fs12). This protein can bind to activin dimer and form a stable complex containing two Fs12 molecules and one activin dimer. We have solved crystal structures of activin A alone and its complex with Fs12 fragment to 2 A resolution. The complex structure shows how Fs12 molecules wrap around the back of the 'wings' of activin, blocking the type II receptor-binding site on activin A. Arginine 192 in Fs2 is a key residue in this interaction, inserting itself in between activin's fingers. Complex formation imposes a novel orientation for the EGF- and Kazal-like subdomains in the Fs2 domain and activin A shows further variation from the canonical TGF-beta family fold. The structure provides a detailed description of the inhibitory mechanism and gives insights into interactions of follistatin with other TGF-beta family proteins.

MeSH Terms
Activin Receptors, Type II/metabolism Activins/antagonists & inhibitors,genetics,metabolism Amino Acid Sequence Animals Binding Sites Crystallography, X-Ray Dimerization Embryo, Mammalian/cytology,metabolism Embryo, Nonmammalian Follistatin/chemistry,genetics,metabolism Humans Inhibin-beta Subunits/antagonists & inhibitors,genetics,metabolism Ligands Models, Molecular Molecular Sequence Data Mutation/genetics Protein Binding Protein Structure, Tertiary Rats Sequence Homology, Amino Acid Signal Transduction Transforming Growth Factor beta/metabolism Xenopus laevis
Chemicals
Follistatin Ligands Transforming Growth Factor beta activin A Activins Inhibin-beta Subunits Activin Receptors, Type II
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Harrington Adrian E
Department of Biochemistry, University of Cambridge, Cambridge, UK.
Morris-Triggs Samantha A
Ruotolo Brandon T
Robinson Carol V
Ohnuma Shin-Ichi
Hyvönen Marko
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
2006-03-08
Epub
2006-00-16
Pages
1035-45
Language
English
Region
England
NLM ID
8208664
PMCID
PMC1409725
Subset
IM
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