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题目:: Modeled structure of the whole regulator G-protein signaling-2.
作者:: Tikhonova(Irina G),Boulègue(Cyril),Langer(Ingrid),Fourmy(Daniel)
状态:: 发布时间2006-02-06 , 更新时间 2006-11-15
期刊:: Biochem Biophys Res Commun
摘要:: There is an increasing interest towards the mechanism by which regulators of G-protein signaling regulate signals of G-protein-coupled receptors. RGS2 is a regulator of Gq protein signaling (RGS), the N-terminal region of which is known to contain determinants for G protein-coupled receptor recognition, but its structure is still unknown. To understand the molecular basis for this recognition, the three-dimensional model of RGS2, including N-terminal region and RGS box, was modeled. For this, RGS4 box structure and data from circular dichroism study of RGS2 N-terminal region were used. Then, membrane-targeting activity of the RGS2 amphipathic helix contained in the N-terminal region was investigated. Furthermore, in cellulo study provided first evidence that an internal sequence within the N-terminal region of RGS2 is involved in RGS2 regulation of cholecystokinin receptor-2 signal. RGS2 modeled structure can now serve to study molecular recognition of RGS2 by signaling molecules.
语言:: eng
DOI:: 10.1016/j.bbrc.2005.12.221

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