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PMID: 16431987 Published · ppublish English Journal Article Research Support, N.I.H., Extramural

Identification and characterization of RsmE, the founding member of a new RNA base methyltransferase family.

RNA (New York, N.Y.) ·Vol. 12 ·No. 3 ·2006-03-00 ·Pages 426-34

Basturea GN, Rudd KE, Deutscher MP

Abstract

A variety of RNA methyltransferases act during ribosomal RNA maturation to modify nucleotides in a site-specific manner. However, of the 10 base-methylated nucleotides present in the small ribosomal subunit of Escherichia coli, only three enzymes responsible for modification of four bases are known. Here, we show that the protein encoded by yggJ, a member of the uncharacterized DUF558 protein family of predicted alpha/beta (trefoil) knot methyltransferases is responsible for methylation at U1498 in 16S rRNA. The gene is well-conserved across bacteria and plants, and likely performs the same function in other organisms. A yggJ deletion strain lacks the methyl group at U1498 as well as the specific methyltransferase activity. Moreover, purified recombinant YggJ specifically methylates m3U1498 in vitro. The deletion strain was unaffected in exponential growth in rich or minimal media at multiple temperatures, but it was defective when grown in competition with isogenic wild-type cells. Based on these data, we conclude that yggJ is the founding member of a family of RNA base methyltransferases, and propose that it be renamed rsmE.

MeSH Terms
Amino Acid Sequence Base Sequence Cloning, Molecular Escherichia coli/enzymology,genetics,growth & development Gene Deletion Genes, Bacterial Genetic Complementation Test Methylation Molecular Sequence Data Phylogeny RNA, Bacterial/chemistry,genetics,metabolism RNA, Ribosomal, 16S/chemistry,genetics,metabolism Sequence Homology, Amino Acid Terminology as Topic tRNA Methyltransferases/chemistry,genetics
Chemicals
RNA, Bacterial RNA, Ribosomal, 16S tRNA Methyltransferases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Basturea Georgeta N
Department of Biochemistry and Molecular Biology, University of Miami Miller School of Medicine, Miami, FL 33101-6129, USA.
Rudd Kenneth E
Deutscher Murray P
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Article Info
Journal
RNA (New York, N.Y.)
Abbr.
RNA
ISSN
1355-8382
Published
2006-03-00
Epub
2006-00-23
Pages
426-34
Language
English
Region
United States
NLM ID
9509184
PMCID
PMC1383581
Subset
IM
Grants
NIGMS NIH HHS · R01 GM058879 · United States
NIGMS NIH HHS · GM58879 · United States
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