Abstract
A variety of RNA methyltransferases act during ribosomal RNA maturation to modify nucleotides in a site-specific manner. However, of the 10 base-methylated nucleotides present in the small ribosomal subunit of Escherichia coli, only three enzymes responsible for modification of four bases are known. Here, we show that the protein encoded by yggJ, a member of the uncharacterized DUF558 protein family of predicted alpha/beta (trefoil) knot methyltransferases is responsible for methylation at U1498 in 16S rRNA. The gene is well-conserved across bacteria and plants, and likely performs the same function in other organisms. A yggJ deletion strain lacks the methyl group at U1498 as well as the specific methyltransferase activity. Moreover, purified recombinant YggJ specifically methylates m3U1498 in vitro. The deletion strain was unaffected in exponential growth in rich or minimal media at multiple temperatures, but it was defective when grown in competition with isogenic wild-type cells. Based on these data, we conclude that yggJ is the founding member of a family of RNA base methyltransferases, and propose that it be renamed rsmE.
MeSH Terms
Amino Acid Sequence
Base Sequence
Cloning, Molecular
Escherichia coli/enzymology,genetics,growth & development
Gene Deletion
Genes, Bacterial
Genetic Complementation Test
Methylation
Molecular Sequence Data
Phylogeny
RNA, Bacterial/chemistry,genetics,metabolism
RNA, Ribosomal, 16S/chemistry,genetics,metabolism
Sequence Homology, Amino Acid
Terminology as Topic
tRNA Methyltransferases/chemistry,genetics
Chemicals
RNA, Bacterial
RNA, Ribosomal, 16S
tRNA Methyltransferases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Basturea Georgeta N
Department of Biochemistry and Molecular Biology, University of Miami Miller School of Medicine, Miami, FL 33101-6129, USA.
Rudd Kenneth E
Deutscher Murray P
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