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PMID: 16426569 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

ST2 suppresses IL-6 production via the inhibition of IkappaB degradation induced by the LPS signal in THP-1 cells.

Biochemical and biophysical research communications ·Vol. 341 ·No. 2 ·2006-03-10 ·Pages 425-32

Takezako N, Hayakawa M, Hayakawa H, Aoki S, Yanagisawa K, Endo H, Tominaga S

Abstract

LPS induces the production of inflammatory cytokines via the stimulation of Toll-like receptors. In this study, we demonstrated that a soluble secreted form of the ST2 gene product (ST2), a member of the interleukin-1 receptor family, suppressed the production of IL-6 in an LPS-stimulated human monocytic leukemia cell line, THP-1. Immunofluorescence confocal microscopy revealed the binding of ST2 to the surface of the THP-1 cells, in which ST2 led to decreased binding of nuclear factor-kappaB to the IL-6 promoter. Furthermore, the degradation of IkappaB in the cytoplasm after LPS stimulation was reduced by pretreatment with ST2. These results demonstrated that ST2 negatively regulates LPS-induced IL-6 production via the inhibition of IkappaB degradation in THP-1 cells.

MeSH Terms

Blotting, Western Cell Line Cell Nucleus/metabolism Cytoplasm/metabolism DNA, Complementary/metabolism Enzyme-Linked Immunosorbent Assay Gene Expression Regulation Humans I-kappa B Proteins/metabolism Immunoprecipitation Interleukin-1/metabolism Interleukin-1 Receptor-Like 1 Protein Interleukin-6/biosynthesis,metabolism Lac Operon Lipopolysaccharides/metabolism Membrane Proteins/physiology Microscopy, Confocal Microscopy, Fluorescence Models, Statistical Monocytes/metabolism NF-KappaB Inhibitor alpha Plasmids/metabolism Promoter Regions, Genetic Protein Binding Receptors, Cell Surface Recombinant Proteins/chemistry Reverse Transcriptase Polymerase Chain Reaction Silver Staining Toll-Like Receptor 4/metabolism Transfection Tumor Necrosis Factor-alpha/metabolism

Chemicals

DNA, Complementary I-kappa B Proteins IL1RL1 protein, human Interleukin-1 Interleukin-1 Receptor-Like 1 Protein Interleukin-6 Lipopolysaccharides Membrane Proteins NFKBIA protein, human Receptors, Cell Surface Recombinant Proteins TLR4 protein, human Toll-Like Receptor 4 Tumor Necrosis Factor-alpha NF-KappaB Inhibitor alpha

Authors & Affiliations

7 authors, click to expand affiliations / ORCID

Takezako Naoki

Department of Biochemistry, Jichi Medical School, 3311-1 Yakushiji, Minamikawachi-machi, Kawachi-gun, Tochigi 329-0498, Japan.

Hayakawa Morisada

Hayakawa Hiroko

Aoki Shinsuke

Yanagisawa Ken

Endo Hitoshi

Tominaga Shin-ichi

Article Info

Journal

Biochemical and biophysical research communications

Abbr.

Biochem Biophys Res Commun

ISSN

0006-291X

DOI

10.1016/j.bbrc.2005.12.206

Published

2006-03-10

Epub

2006-00-11

Pages

425-32

Language

English

Region

United States

NLM ID

0372516

Subset

External Links

PubMed source DOI: 10.1016/j.bbrc.2005.12.206 View journal details

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ST2 suppresses IL-6 production via the inhibition of IkappaB degradation induced by the LPS signal in THP-1 cells.

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